Glucose-6-phosphate dehydrogenase deficiency genotypes and allele frequencies in the Kavango and Zambezi regions of northern Namibia
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Date
2019-04-09
Journal Title
Journal ISSN
Volume Title
Publisher
Transactions of the Royal Society of Tropical Medicine and Hygiene
Abstract
Background: Namibia has made significant gains in the fight against malaria, with a target of elimination by
2023. We examined the genotype and allele frequencies of glucose-6-phosphate dehydrogenase (G6PD) deficiency
to inform decisions on primaquine use, as we recently detected clusters of Plasmodium ovale curtisi in
Kavango.
Methods: A multistaged cross-sectional sampling method was used to enrol 212 children 2–9 y of age from
schools and clinics in the Okavango and Zambezi regions of northern Namibia. Genotypes for the 202 G→A
and 376 A→G mutations were assigned by polymerase chain reaction restriction fragment length
polymorphism.
Results: Of the 212 subjects enrolled, genotypes were available for 210, made up of 61 males and 149 females.
G6PD-deficient males (hemizygotes) and females (homozygotes) constituted 3.27% (2/61) and 0.0% (0/149),
respectively. Female heterozygotes (AA− and BA−) constituted 10.07% (15/149), while G6PD wild-type males
(with A or B haplotype) and females (with AA, BB or AB haplotypes) consisted of 96.72% (59/61) and 89.93%
(134/149), respectively. The A−, A and B allele frequencies were 0.0474, 0.3036 and 0.6490, respectively.
Hardy–Weinberg equilibrium tests for female genotype frequencies did not show deviation (p=0.29).
Conclusions: The frequency of G6PD deficiency alleles in males in the Kavango and Zambezi regions of northern
Namibia constitute 3.27%, a first report to inform policy on primaquine role out.
Description
Research Article
Keywords
G6PDd alleles, Genotypes, Kavango, Namibia, Primaquine, Zambezi