Association between global DNA methylation (LINE-1) and occupational particulate matter exposure among informal electronic-waste recyclers in Ghana
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Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor & Francis Group
Abstract
This study examined the associations between PM (2.5 and 10) and global
DNA methylation among 100 e-waste workers and 51 non-e-waste work ers serving as controls. Long interspersed nucleotide repetitive elements 1 (LINE-1) was measured by pyrosequencing. Personal PM2.5 and PM10
were measured over a 4-hour work-shift using real-time particulate moni tors incorporated into a backpack . Linear regression models were used to
assess the association between PM and LINE-1 DNA methylation. The
concentrations of PM2.5 and PM10 were significantly higher among the
e-waste workers than the controls (77.32 vs 34.88, p < 0.001 and 210.21 vs
121.92, p < 0.001, respectively). PM2.5 exposure was associated with
increased LINE-1 CpG2 DNA methylation (β = 0.003; 95% CI; 0.001, 0.006;
p = 0.022) but not with the average of all 4 CpG sites of LINE-1. In
summary, high levels of PM2.5 exposure was associated with increased
levels of global DNA methylation in a site-specific manner.
Description
Research Article
Keywords
DNA methylation, LINE-1, PM2.5, PM10, e-waste, e-waste workers