Proteolytic Activity In Adult Haemonchus Contortos
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Date
1982
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Publisher
University of Ghana
Abstract
Proteolytic activity of the JQ.qOOxgsupernate of
homegenates ef adult Haemonchus contortus has been
studied with respect to substrates susceptible to its
proteolytic activity, and the effects of factors such as
temperature, pH, substrate concentration, extract concentration,
dibasic metal ions, detergents, heat and some
specific inhibitors of proteolytic activity.
The supernate had two pH optima, 5.6 with haemoglobii
as substrate, and 7.8 using L-alanyl - p-nitroanilide.
It showed optimum activity at 36°C, was heat labile and
in general was not significantly affected by the presence
of metal ions in the hydrolysis of haemoglobin and L-alanyl
p-nitroanilide. In screening for substrates susceptible
to its hydrolytic action the most suitable were found to
be those p-nitroanilides and jp-naphthylamides which have a
single amino acid group (small R-group), unprotected at
the free functional group end.
The kinetic studies indicated that haemoglobin was
more rfeadily hydrolysed than the p-nitroanilides and
p-naphthylamides.
Description
Thesis(MSc)-University of Ghana,1982.