Immunological Characterization Of A Novel Merozoite Surface Protein (PFMSP11)
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Date
2020-12
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University Of Ghana
Abstract
Background: Plasmodium falciparum merozoite surface protein 11 (PfMSP11) is a novel surface protein that plays a critical role during the parasite’s blood stage development. This project aimed at immunologically characterizing the Plasmodium falciparum Merozoite Surface protein (PfMSP11).
Method: Antibody (IgG) responses from 283 child clinical malaria samples Accra, Kintampo and Navrongo which represents different transmission intensities, were measured against the 20 synthetic peptides of PfMSP1 using ELISA. Overall 27 synthetic peptides were tested against PBMCs from 10 apparently healthy subjects to measure IFN-γ responses using ELISpot.
Results: Peptides at the C-terminus of PfMSP11 gave higher antibody responses than peptides at the N-terminus. Peptide 2 to Peptide 12, and Peptide 17 recorded low antibody responses across all sites. Peptide 16 recorded the highest antibody responses across all sites. One subject (M14) showed a highest response across all pools in measuring IFN-γ responses . Subject M9 tested positive for pools 1 and 3 while subject M10 and M12 tested positive for pool 2Peptide 7 in pool 2 against subject M10 was the only peptide that tested positive.
In conclusion, this study has shown that there are more antibody epitopes at the C-terminus than the N-terminus and more immunogenic T cell epitopes at the N-terminus.
Description
MPhil. Biochemistry
Keywords
Plasmodium, Plasmodium falciparum, Merozoite Surface Protein