Selective activation of TCR-γδ+ cells in endemic burkitt's lymphoma.
| dc.contributor.author | Futagbi, G. | |
| dc.contributor.author | Welbeck, J.E. | |
| dc.contributor.author | Tetteh, J.K. | |
| dc.contributor.author | Hviid, L. | |
| dc.contributor.author | Akanmori, B.D. | |
| dc.date.accessioned | 2013-06-14T18:13:25Z | |
| dc.date.accessioned | 2017-10-19T11:48:42Z | |
| dc.date.available | 2013-06-14T18:13:25Z | |
| dc.date.available | 2017-10-19T11:48:42Z | |
| dc.date.issued | 2007-05-23 | |
| dc.description.abstract | BACKGROUND: The overlap in geographical distribution of Plasmodium falciparum malaria and endemic Burkitt's lymphoma (eBL)--an aggressive Epstein-Barr virus (EBV)-associated B-cell tumour occurring almost exclusively in the tropics--strongly suggests a link between the two diseases. It is suspected that the polyclonal B-cell activation in P. falciparum malaria may precipitate a breakdown in homeostatic T-cell control of EBV-immortalized B-cell proliferation. Previous studies have suggested that a particular T-cell subset, characterized by expression of Vdelta1+ gammadelta T-cell receptors, is important for maintaining B-cell homeostasis, both in P. falciparum- exposed populations and in individuals subject to polyclonal B-cell activation of other aetiology. The objective of the present study was, therefore, to characterize lymphocyte phenotypes and to investigate possible differences in T-cell subset composition and activation status in P. falciparum-exposed Ghanaian children with and without eBL. METHODS: Venous blood samples in heparin from 21 eBL patients (mean age: 7.0 years; range: 3-11 years), referred to the Burkitt's Tumour Centre at Korle-Bu Teaching Hospital, Accra and 15 healthy, age and sex matched children, were stained with fluorescein isothiocyanate (FITC)-, phycoerythrin (PE)-, R-phycoerythrin (RPE)- and RPE-Cy5-conjugated antibodies (CD3, CD4, CD8, CD25, CD69, CD95, HLA-DR, TCR-gammadelta, Vdelta1, Vdelta3, Vgamma9 and B-cells) and acquired on a flow cytometer. RESULTS: A reduction in the proportion of CD3+ cells in eBL patients, due mainly to perturbations among TCR-gammadelta+ cells was observed. In contrast, the proportions of CD4+ or CD8+ cells were relatively unaffected, as were the mean numbers of peripheral blood mononuclear cells. CONCLUSION: Selective changes in numbers and activation status of TCR-gammadelta+ cells occurs in Ghanaian children with eBL, a pattern which is similar to P. falciparum-induced changes. The data supports the hypothesis of a regulatory role for Vdelta1+ TcR-gammadelta T-cells in maintaining B-cell homeostasis and provides insights into the pathogenesis of eBL. | en_US |
| dc.identifier.issn | PMID: 17521425 | |
| dc.identifier.uri | http://197.255.68.203/handle/123456789/3202 | |
| dc.language.iso | en | en_US |
| dc.publisher | PubMed | en_US |
| dc.title | Selective activation of TCR-γδ+ cells in endemic burkitt's lymphoma. | en_US |
| dc.type | Article | en_US |