Observation of unphosphorylated STAT3 core protein binding to target dsDNA by PEMSA and X-ray crystallography
| dc.contributor.author | Nkansah, E | |
| dc.contributor.author | Shah, R | |
| dc.contributor.author | Collie, G.W. | |
| dc.contributor.author | Parkinson, G.N. | |
| dc.contributor.author | Palmer, J | |
| dc.contributor.author | Rahman, K.M. | |
| dc.contributor.author | Wilderspin, A.F. | |
| dc.date.accessioned | 2013-06-21T13:18:35Z | |
| dc.date.accessioned | 2017-10-16T11:43:51Z | |
| dc.date.available | 2013-06-21T13:18:35Z | |
| dc.date.available | 2017-10-16T11:43:51Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | The STAT3 transcription factor plays a central role in a wide range of cancer types where it is over-expressed. Previously, phosphorylation of this protein was thought to be a prerequisite for direct binding to DNA. However, we have now shown complete binding of a purified unphosphorylated STAT3 (uSTAT3) core directly to M67 DNA, the high affinity STAT3 target DNA sequence, by a protein electrophoretic mobility shift assay (PEMSA). Binding to M67 DNA was inhibited by addition of increasing concentrations of a phosphotyrosyl peptide. X-ray crystallography demonstrates one mode of binding that is similar to that known for the STAT3 core phosphorylated at Y705 | en_US |
| dc.identifier.citation | Nkansah, E., Shah, R., Collie, G. W., Parkinson, G. N., Palmer, J., Rahman, K. M., . . . Wilderspin, A. F. (2013). Observation of unphosphorylated STAT3 core protein binding to target dsDNA by PEMSA and X-ray crystallography. FEBS Letters | en_US |
| dc.identifier.uri | http://197.255.68.203/handle/123456789/3824 | |
| dc.language.iso | en | en_US |
| dc.publisher | FEBS Letters | en_US |
| dc.title | Observation of unphosphorylated STAT3 core protein binding to target dsDNA by PEMSA and X-ray crystallography | en_US |
| dc.type | Article | en_US |