Reservoir of Antibiotic Residues and Resistant Coagulase Negative Staphylococci in a Healthy Population in the Greater Accra Region, Ghana

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dc.contributor.authorBekoe, S.O.
dc.contributor.authorHane-Weijman, S.
dc.contributor.authorTrads, S.L.
dc.contributor.authorOrman, E.
dc.contributor.authorOpintan, J.
dc.contributor.authorHansen, M.
dc.contributor.authorFrimodt-Møller, N.
dc.contributor.authorStyrishave, B.
dc.date.accessioned2022-02-07T15:53:04Z
dc.date.available2022-02-07T15:53:04Z
dc.date.issued2022
dc.descriptionResearch Articleen_US
dc.description.abstractAntimicrobial resistance threatens infectious disease management outcomes, especially in developing countries. In this study, the occurrence of resistant coagulase-negative staphylococci (rCoNS) and antibiotic residues in urine samples of 401 healthy individuals from Korle-Gonno (KG) and Dodowa (DDW) in Ghana was investigated. MALDI-ToF/MS with gram-staining techniques detected and identified the CoNS. SPE-LC-MS/MS detected and quantified nine commonly used antibiotics in the samples. The results showed 63 CoNS isolates detected in 47 (12%) samples, with S. haemolyticus (78%) and S. epidermidis (8%) being predominant. Most of the isolates (95%) were resistant to at least one antibiotic, with the highest resistance observed against sulphamethoxazole (87%). Resistance profiles in samples from DDW and KG were largely comparable, but with some differences. For instance, DDW isolates were more resistant to gentamicin (p = 0.0244), trimethoprim (p = 0.0045), and cefoxitin (p = 0.0078), whereas KG isolates were more resistant to erythromycin (p = 0.0356). Although the volunteers had not knowingly consumed antibiotics two weeks before sampling, antibiotic residues, ranging between 1.44–17000 ng mL−1 were identified in 22% of urine samples. Samples with antibiotic residues were likely to also contain rCoNS (89%). The most frequent antibiotics detected were tetracycline (63%) and ciprofloxacin (54%). Healthy individuals could thus be reservoirs of antibiotic residues and rCoNS at the community level.en_US
dc.identifier.otherhttps://doi.org/10.3390/antibiotics11010119
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/37807
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subjectantimicrobial resistanceen_US
dc.subjectresistant CoNSen_US
dc.subjecthealthy individualsen_US
dc.subjectLC-MS/MSen_US
dc.subjectantimicrobial susceptibility testen_US
dc.titleReservoir of Antibiotic Residues and Resistant Coagulase Negative Staphylococci in a Healthy Population in the Greater Accra Region, Ghanaen_US
dc.typeArticleen_US

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