Rapid and sensitive detection of mycobacterium ulcerans by use of a loop-mediated isothermal amplification test
dc.contributor.author | Njiru, Z.K. | |
dc.contributor.author | Yeboah-Manu, D. | |
dc.contributor.author | Stinear, T.P. | |
dc.contributor.author | Fyfe, J.A.M. | |
dc.date.accessioned | 2013-06-17T08:43:29Z | |
dc.date.accessioned | 2017-10-16T13:19:28Z | |
dc.date.available | 2013-06-17T08:43:29Z | |
dc.date.available | 2017-10-16T13:19:28Z | |
dc.date.issued | 2012 | |
dc.description.abstract | This work reports the design and evaluation of a rapid loop-mediated isothermal amplification test for detecting Mycobacterium ulcerans DNA based on the multicopy insertion sequence IS2404. The test is robust and specific with a detection limit equivalent to 20 copies of the target sequence (0.01 to 0.1 genome). The test has potential for the diagnosis of Buruli ulcer under field conditions | en_US |
dc.identifier.citation | Njiru, Z. K., Yeboah-Manu, D., Stinear, T. P., & Fyfe, J. A. M. (2012). Rapid and sensitive detection of mycobacterium ulcerans by use of a loop-mediated isothermal amplification test. Journal of Clinical Microbiology, 50(5), 1737-1741. | en_US |
dc.identifier.uri | http://197.255.68.203/handle/123456789/3245 | |
dc.language.iso | en | en_US |
dc.publisher | Journal of Clinical Microbiology | en_US |
dc.title | Rapid and sensitive detection of mycobacterium ulcerans by use of a loop-mediated isothermal amplification test | en_US |
dc.type | Article | en_US |
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