Rapid and sensitive detection of mycobacterium ulcerans by use of a loop-mediated isothermal amplification test
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Date
2012
Authors
Njiru, Z.K.
Yeboah-Manu, D.
Stinear, T.P.
Fyfe, J.A.M.
Journal Title
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Volume Title
Publisher
Journal of Clinical Microbiology
Abstract
This work reports the design and evaluation of a rapid loop-mediated isothermal amplification test for detecting Mycobacterium ulcerans DNA based on the multicopy insertion sequence IS2404. The test is robust and specific with a detection limit equivalent to 20 copies of the target sequence (0.01 to 0.1 genome). The test has potential for the diagnosis of Buruli ulcer under field conditions
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Citation
Njiru, Z. K., Yeboah-Manu, D., Stinear, T. P., & Fyfe, J. A. M. (2012). Rapid and sensitive detection of mycobacterium ulcerans by use of a loop-mediated isothermal amplification test. Journal of Clinical Microbiology, 50(5), 1737-1741.