Effects of factor v Leiden polymorphism on the pathogenesis and outcomes of preeclampsia
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Date
2019-11-27
Journal Title
Journal ISSN
Volume Title
Publisher
BMC Medical Genetics
Abstract
Background: Factor V Leiden polymorphism is a well-recognized genetic factor in the etiology of preeclampsia.
Considering that Ghana is recording high incidence of preeclampsia, we examined if factor V Leiden is a
contributory factor to its development and pregnancy outcomes.
Methods: STROBE consensus checklist was adopted to recruit eighty-one (81) consenting subjects after ethical
clearance. Subjects were followed up till delivery to obtain outcomes of PE. Routine blood chemistry and
proteinuria were done on all samples. Factor V Leiden was characterized by polymerase chain reaction and
restriction fragment length polymorphism (RFLP). The data was captured as protected health information (PHI) and
analyzed with SPSS version 22.
Results: Overall allelic frequencies found in FVL exon 10 were 0.67 and 0.33 for G and A alleles respectively. The
FVL mutation was more in PE and hypertensive patients. Increased white blood cells, increased uric acid and a
three – fold increment of AST / ALT ratio was observed in PE cases when stratified by FVL exons (exon 8 and 10).
Significant differences were also observed between FVL and age, systolic blood pressure (SBP), diastolic blood
pressure (DBP), liver enzymes, white blood cells (wbc), hemoglobin levels.
Conclusion: FVL mutation allele frequency was 0.33, a first report. The mutation was associated with increased uric
acid, liver enzymes and blood cell indices suggestive of acute inflammation.
Description
Research Article
Keywords
Factor V, Leiden, Preeclampsia, Polymerase chain reaction, Restriction