T7 expression plasmids for producing a recombinant human G1P[8] rotavirus comprising RIX4414 sequences of the RV1 (Rotarix , GSK) vaccine strain
Date
2023
Journal Title
Journal ISSN
Volume Title
Publisher
Microbiology Resource Announcements
Abstract
The live oral rotavirus RV1 (Rotarix) vaccine is formulated from the human
G1P[8] RIX4414 virus. Based on RIX4414 sequences, T7 expression plasmids were
constructed that supported recovery of recombinant RIX4414-like viruses by reverse
genetics. These plasmids will advance the study of the RV1 vaccine, possibly allowing
improvements to its efficacy.
Description
Research Article
Keywords
reverse genetic analysis, vaccines, rotavirus