Effect of Qhloroquine on the Induction of Rat Uterine Peroxidase by an Oral Contraceptive
Date
1979-12
Authors
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Publisher
University of Ghana
Abstract
Uterine peroxidase has been induced in immature female rate
with estradiol and anovlar 21. Anovlar 21 is a steroid contraceptive
containing 4 mg. norethisterone acetate (a progestin) , and 0.OS mg.
ethinyl estradiol (an estrogen) per tablet. Maximum enzyme aettpity
was found to occur 24 hours a fter oral adminis tration o f the contraceptive.
The increased peroxidase activity caused by the cotrbined
steroids was inhibited by the administration o f cyclohexirrrLde and
puromycin (inhibitors o f protein biosgitheais). Peroxidase is thus
synthesised ok novo on the influence o f the steroids. Chloroquine diphosphate has also been found to increase the
activity o f peroxidase, with the maximum activity occtoMng 18 hours
after the drug has been injected intramuscularly. Cycloheximide has
been found to prevent this stimulatory effect o f chloroquine. Chloroquine
was effective in increasing peroxidase activity in vivo over a
dose range o f 0-20 mg/kg. body weight. I t however± did not affect
peroxidase activity in vitro. Chloroquine potentiated uterine peroxidase induction by anovlar 21.
Chloroquine and/or anovlar 21 prevented the production o f litte r by
adult female rats.
Description
Thesis(M.Phil)-University of Ghana, 1979