Possible adverse effect of high δ-alpha-tocopherol intake on hepatic iron overload: enhanced production of Vitamin C and the genotoxin, 8-hydroxy-2’-dexyguanosine
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Date
2010
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Toxicology Mechanisms and Methods 20(2): 96-104
Abstract
Excess hepatic iron generates reactive oxygen species that result
in oxidative stress and oxidative damage to the layer. Vitamins have hitherto been considered to be a possible remedy. The aim of this study was to determine if high doses of δ-a-tocopherol supplementation in iron overload would ameltorate the oxidative stress. Four groups of 20 males Wistar albino rats were studied: group 1 (control) was fed normal diet, group 2 (Fe) 0.75% Ferrocene iron, group 3 (FV gp) 0.75% Ferrocene/δ-a-tocopherol (10x RDA), group 4 (V gp) normal diet/δ-a-tocopherol. After 12 months, serum iron, reduced glutathione, catalase, Vitamin C., Oxygen Radical Absorbance Capacity, lipid peroxidation, 8-hydroxy-2’ dexyguanosine (8-OHdG), aspartate transaminase (AST), and alanine transaminase (ALT) were measured. Vitamin C levels were F gp=5.85 ± 0.13 (µmol/l) (p<0.05). 8-hydroxy 2’-dexyguanosine levels were F gp – 143.6 ± 6.4; FV gp – 179.2 ± 18.2 (ng/ml) (p,0.05). Oxidative liver damage, as determined by serum AST and ALT levels, was not attenuated by a-tocopherol. A positive correlation existed between vitamin C and 8-OHdg, suggesting possible a-tocopherol toxicity.
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Keywords
a-Tocopherol, antioxidants, 8-hydroxy-2’-dexyguanosine, iron overload