Browsing by Author "Rozek, L.S."
Now showing 1 - 2 of 2
Results Per Page
Sort Options
Item Association between global DNA methylation (LINE-1) and occupational particulate matter exposure among informal electronic-waste recyclers in Ghana(Taylor & Francis Group, 2021) Issah, I.; Arko-Mensah, J.; Rozek, L.S.; Rentschler, K.; Agyekum, T.P.; Dwumoh, D.; Batterman, S.; Robins, T.G.; Fobil, J.N.This study examined the associations between PM (2.5 and 10) and global DNA methylation among 100 e-waste workers and 51 non-e-waste work ers serving as controls. Long interspersed nucleotide repetitive elements 1 (LINE-1) was measured by pyrosequencing. Personal PM2.5 and PM10 were measured over a 4-hour work-shift using real-time particulate moni tors incorporated into a backpack . Linear regression models were used to assess the association between PM and LINE-1 DNA methylation. The concentrations of PM2.5 and PM10 were significantly higher among the e-waste workers than the controls (77.32 vs 34.88, p < 0.001 and 210.21 vs 121.92, p < 0.001, respectively). PM2.5 exposure was associated with increased LINE-1 CpG2 DNA methylation (β = 0.003; 95% CI; 0.001, 0.006; p = 0.022) but not with the average of all 4 CpG sites of LINE-1. In summary, high levels of PM2.5 exposure was associated with increased levels of global DNA methylation in a site-specific manner.Item Global DNA (LINE‑1) methylation is associated with lead exposure and certain job tasks performed by electronic waste workers(International Archives of Occupational and Environmental Health, 2021) Issah, I.; Arko‑Mensah, J.; Rozek, L.S.; Zarins, K.R.; Agyekum, T.P.; Dwomoh, D.; Basu, N.; Batterman, S.; Robins, T.G.; Fobil, J.N.Objective This study assessed the associations between blood and urine levels of toxic metals; cadmium (Cd) and lead (Pb), and methylation levels of the LINE-1 gene among e-waste and control populations in Ghana. Methods The study enrolled 100 male e-waste workers and 51 all-male non-e-waste workers or controls. The concentrations of Cd and Pb were measured in blood and urine using inductively coupled plasma mass spectrometry, while LINE1 methyla tion levels were assessed by pyrosequencing of bisulfte-converted DNA extracted from whole blood. Single and multiple metals linear regression models were used to determine the associations between metals and LINE1 DNA methylation. Results Blood lead (BPb) and urine lead (UPb) showed higher median concentrations among the e-waste workers than the controls (76.82 µg/L vs 40.25 µg/L, p≤0.001; and 6.89 µg/L vs 3.43 µg/L, p≤0.001, respectively), whereas blood cadmium (BCd) concentration was lower in the e-waste workers compared to the controls (0.59 µg/L vs 0.81 µg/L, respectively, p=0.003). There was no signifcant diference in LINE1 methylation between the e-waste and controls (85.16±1.32% vs 85.17±1.11%, p=0.950). In our single metal linear regression models, BPb was signifcantly inversely associated with LINE1 methylation in the control group (βBPb=− 0.027, 95% CI − 0.045, − 0.010, p=0.003). In addition, a weak associa tion between BPb and LINE1 was observed in the multiple metals analysis in the e-waste worker group (βBPb=− 0.005, 95% CI − 0.011, 0.000, p=0.058). Conclusion Continuous Pb exposure may interfere with LINE1 methylation, leading to epigenetic alterations, thus serving as an early epigenetic marker for future adverse health outcomes.