Browsing by Author "Bukari, Y."
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Item In vitro activity of soybean extract on cocoa disease pathogens (Erythricium salmonicolor and Marasmiellus scandens) and termite pest (Microtermes subhyalinus)(Cogent Food & Agriculture, 2022) Bukari, Y.; Avicor, S.W.; Awudzi, G.; Ainooson, M.K.; Eric K. AsareThis study evaluated the antifungal and insecticidal properties of soybean extract on Marasmiellus scandens (causal pathogen of white thread blight disease on cocoa), Erythricium salmonicolor (causal pathogen of pink disease of cocoa) and termite (Microtermes subhyalinus). Agar plate test was used to determine the inhibitory activity of the extract on mycelial growth of the pathogens while a contact toxicity bioassay was conducted on the termites. Extract of the soybean reduced mycelial growth in a dose-dependent pattern, with 20% of the extract attaining the highest inhibition. Percentage inhibition of 80.4 and 86.8 were recorded for E. salmonicolor and M. scandens, respectively after 7 days of incuba tion. The IC50 values ranged between 0.64–2.44% and 0.12–0.45% for E. salmonicolor and M. scandens respectively. However, activity of the extract on the termite was low, with an LC50 of 15.42% after 24 hours of exposure. The effect of the extract on the fungal pathogens indicates that it could be further explored towards the development of a plant-based control method against white thread blight and pink diseases of cocoa in Ghana.Item Survival of Mushrooms and Termites Upon Pesticide Exposure in the Cocoa Agro-ecosystem(JOURNAL OF TROPICAL LIFE SCIENCE, 2023) Asare, E.K.; Avicor, S.W.; Bukari, Y.; Amoako-Attah, I.; Agyare, S.; Yeboah, M.; Wiafe-Kwagyan, M.Pesticides have become integral parts of cocoa cultivation for the management of in sect pests and fungal pathogens which cause significant damage to the crop. However, continuous pesticide usage in the cocoa agro-ecosystem is of concern due to perceived adverse effects on non-target organisms. In this study, mushrooms and associated ter mites were used to elucidate the possible effect of fungicides and insecticides on non target organisms in the cocoa agro-ecosystem. The vegetative phase of Pleurotus sa jor-caju (Oyster mushroom), Volvariella volvacea (Paddy straw mushroom), Termi tomyces globulus, and Termitomyces robustus (Termite mushrooms) were subjected to concentrations of commercially formulated fungicides (metalaxyl 12 % + copper (I) oxide 60 % and cupric hydroxide 77 %) and insecticides (imidacloprid 20 % and bifenthrin 2.7 %) to observe their growth rates on Potato Dextrose Agar (PDA). Worker termites, Macrotermes bellicosus, were also exposed to the pesticides in Petri dishes for 24 h to observe their mortality. The manufacturer’s recommended concen tration of 245 ppm for bifenthrin completely inhibited mycelial growth of all the mushrooms and caused 100% mortality of termites. At 0.0245 ppm, the insecticide caused 60% mortality of termites, but it had no inhibitory effect on the mushrooms. Except for P. sajor-caju, mycelial growth of all the other mushrooms was completely inhibited by metalaxyl + copper (I) oxide at the manufacturer’s recommended con centration of 2400 ppm. However, mycelial growth rate of the mushrooms at 0.24 ppm of the fungicide was similar to the control plates. Although the recommended concentrations of the pesticides inhibited mushroom activity under controlled condi tions, mushroom survival in the cocoa agro-ecosystem amidst pesticides could be due to diluted pesticide concentrations that result in the soil after application which is usu ally directed at the pods, trunk, and foliage