Browsing by Author "Amiteye, S."
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Item Efficacy of mixing vegetable oils with pirimiphos-methyl against the maize weevil, Sitophilus zeamais Motschulsky in stored maize(Journal of Stored Products Research, 2005-05) Obeng-Ofori, D.; Amiteye, S.Sitophilus zeamais is a major insect pest of stored maize and grain products in the tropics. The toxicity of coconut, groundnut and soybean oils applied at 1, 2, 5 and 10ml/kg and pirimiphos-methyl at 2.5, 5 and 10μl active ingredient in 2ml of water/kg of grain (116, 18 and 14 of the recommended dose), alone or in combination, to adults and immature stages of S. zeamais, the persistence of the treatments in maize grains and their effects on seed viability were evaluated in the laboratory. All the treatments caused significant mortality compared to untreated controls. Low dosages of the oils and pirimiphos-methyl when combined were highly toxic to adult S. zeamais. The mixtures completely inhibited the development of immature stages of the weevil. Only pirimiphos-methyl and the mixtures retained some activity 60 days after application. Pirimiphos-methyl can be used at reduced rates if combined with vegetable oils to control infestations of S. zeamais in stored maize. © 2004 Elsevier Ltd. All rights reserved.Item Genetic Diversity Studies in Twenty Accessions of Hot Pepper (Capsicum Spp L.) In Ghana.(University of Ghana, 2015-07) Doku, S.K.; Amoatey, H.M.; Amiteye, S.; University of Ghana, College of Basic and Applied Sciences, Department of Nuclear Agriculture and Radiation ProcessingTwenty (20) accessions of hot pepper (Capsicum spp L.) were collected from eight geographical regions of Ghana for genetic diversity studies. The objective was to assess genetic relationship among them using phenotypic and molecular traits and to evaluate their elemental composition. A replicated field experiment was conducted to assess their genetic diversity based on 13 quantative traits and 22 qualitative traits using the IBPGR descriptor list for Capsicum. Confirmation of their identities was done using 10 SSR markers. The accessions were also evaluated for macro, micro and trace elements in their fresh fruits using the Instrumental Neutron Activation Analysis (INAA). Five essential macro elements (Ca, Cl, K, Mg and Na), two micro elements (Al and Mn) and one trace element (Br) were detected by INAA. Results from the agromorphological study revealed that accession Wes 01 had the widest stem width, matured leaf width, high fruit set but late maturing. Nor 03 was early maturing and had high fruit set, but also possesssed the highest number of seeds per fruit. Fruit weight, fruit width, fruit length and plant canopy width, recorded the highest variabilities with 66.191; 53.24; 49.32; and 32.42 coefficients of variation (CVs), respectively. Few traits such as plant canopy width, plant height, fruit length, mature leaf length and number of seeds per fruit contributed substantially to total genetic variance as revealed by the principal component analysis (PCA). A dendrogram generated using morphological traits grouped accessions into cultivated and wild genotypes of pepper and all the accessions were identified as separate entities with no duplications. Strong correlation was recorded between plant canopy width and plant height, mature leaf length and mature leaf width, and also fruit weight and fruit width and fruit length. Negative correlation was however, observed between fruit length and days to 50% fruiting and flowering. All three accessions from the Northern Region, Nor-01, Nor-02 and Nor-03, as well as Vol-01 from the Volta Region recorded high amount of Sodium in their fruit samples. Genetic analysis of the structure of the population showed close resemblance among the accessions with a high genetic identity coefficient of 0.975 between populations 1 and 2. A rather short genetic distance was recorded evidenced by high outcrossing rate of 0.72. Accessions Wes 01 and Nor 03 may be recommended for improvement for the export market.Item Improvement in cowpea variety Videza for traits of extra earliness and higher seed yield(Heliyon, 2022) Amenorpe, G.; Dorvlo, I.K.; Amoatey, H.M.; Amiteye, S.; Kutufam, J.T.; Afutu, E.; Asare-Bediako, E.; Darkwa, A.A.The cowpea variety Videza, which was used as the control, matures early (70 days after planting), although it produces low yields. Gamma irradiation mutagenesis was used to induce Videza into extra-early maturing and higher yielding mutant genotypes. A single seed descend population was developed for radio-sensitivity test, and a Lethal Dose 50 (LD50) of 240.5 Gy was determined, and applied from a cobalt-60 (60Co) source, to acutely mass irradiate 1800 Videza seeds, at the Ghana Atomic Energy Commission. The irradiated seeds (M1) were planted to produce M2 seeds bearing plants and subsequently advanced to M3 plants for selection of nine induced plants based on extra earliness and significantly higher seed yields than the parental control. It took 48 days after planting (DAP) for the genotype coded P1N02#1 to reach 50 % maturity followed by 52 DAP for genotypes with codes P4N03#3; P3N01#5; P5N05#6, P4N14#7, P5N07#8, P5N05#10 and 54 DAP for genotype P4N14#11. P1N06#9 had the highest yield (97.38 g/plant), followed by P5N05#10 (95.97 g/plant), P1N08#13 (81.24 g/ plant), P2N09#12 (73.94 g/plant), P6N10#19 (70.83 g/plant), P1N06#20 (65.36 kg/plant), P5N07#14 (61.23 g/plant), P4N14# (58.05 g/plant) and P1N08#17 (56.23 g/plant). M3 seeds were advanced to M4 plants for a Preliminary Yield Trial which revealed that induced plants P5N05#10 (1235 kg/ha), P2N09#12 (1206 kg/ha), P5N07#14 (1185 kg/ha), P1N06#20 (1171 kg/ha), P1N06#9 (1051 kg/ha), P1N08#13 (1041 kg/ha), and P6N10#19 (999 kg/ha) outperformed the control (517 kg/ha) and two other commercial varieties. Overall, the two highest performing candidates for further evaluation for varietal release were P5N05#10 and P2N09#12.Item Participatory approach of preferred traits, production constraints and mitigation strategies: implications for soybean breeding in Guinea Savannah zone of Ghana(Heliyon, 2022) Addae-Frimpomaah, F.; Amenorpe, G.; Denwar, N.N.; Amiteye, S.; Adazebra, G.A.; Sossah, F.L.; Akaba, S.; Issah, A.R.; Amoatey, H.M.Soybean production is concentrated in the Guinea Savannah agro-ecological zone of Ghana. However, its cultivation is plagued with a number of constraints leading to low yields. A participatory approach was, therefore, used to identify farmers' and processors' preferred soybean traits and production constraints, climate change effects and strategies employed for mitigating these effects in three districts within the soybean growing areas in Northern Ghana. The study revealed that 72.0 % and 68.8 % of farmers in the Tolon and Savelugu districts, respectively, used improved soybean seeds for planting their fields. Over 13.0 % of farmers indicated late maturity as the most important constraint, followed by manual threshing difficulty and terminal drought which results in low grain yield and poor quality. About 70.0 % of farmers opined that climate change effects are causing obvious shifts in the rainfall pattern. Majority of the farmers (94.6 %) indicated that the onset of peak rainfall has changed in the past decade. Almost 44.0% of farmers also indicated that severe drought has affected pods filling in the past. A third (33.3%) of farmers indicated that drought usually sets in at pod initiation. The farmers enumerated some of the effects of early cessation of the rainfall in soybean production as drying up of immature and green seed (28.3%), low grain yield (27.1%) and poor seed quality (22.1%). Some of the mitigation strategies employed by the farmers include early planting (40.0%) and mulching (25.0%) to retain soil moisture for enhanced growth of crops. The order of farmers’ preferences new soybean varieties were shattering resistance (16.0%), high grain yield (14.0%), large seeds size (13.1%), and early maturity (11.8%), whereas processors preferred varieties with large seed size (30.6%), high protein content (28.7%), pest resistance (15.9%) and short cooking duration (12.7%). Both the farmers and processors indicated their willingness to pay more for seeds with the desired traits. These findings will aid soybean breeders in developing new varieties that possess desired traits preferred by both farmers and processors for increased soybean cultivation and utilization.Item Physiological changes associated with Okra mosaic virus infection in field grown okra plants(AJAB, 2021) Amiteye, S.; Appiah, A.S.; Boateng, F.; Kutufam, J.T.; Amoatey, H.M.As a prerequisite for the breeding of virus-resistant okra cultivars, the effect of Okra mosaic virusinfection on physiological performance of ten okra cultivars were assessed under field conditions within the coastal savannah agro-ecological zone of Ghana. All cultivars succumbed to the disease and expressed varying degrees of symptoms. The results showed that plant height at initial and 50% flowering were significantly reduced with cultivar Clemson spineless showing the highest reduction of 38% and 35% respectively. Plant height at 50% podding was also reduced in all cultivars with the highest reduction (40%) occurring in cultivar Clemson spineless. Virus-infected plants of cultivars Kwabenya 1, Labadi dwarf and Asutem took on the average 43 days to attain 50% flowering compared to 25 days in healthy or control plants. Diseased plants had significantly (P<0.05) delayed, taking on the average 47 days after planting to attain 50% podding compared to the control plants with an average of 35 days. Infected plants of cultivars Kwabenya 1 and Labadi dwarf took the highest number of 51 days to attain 50% podding. Pod length and width were also reduced in all cultivars but were highest in cultivars Lucky 19 F1 and Clemson spineless at 52.19% and 46.25% respectively. Based on this assessment, cultivars Adom and Asutem which were less affected in terms of disease severity and fruit yield reduction could be exploited in future breeding programmes.Item The use of Microsatellite Markers in Genetic Variation Analysis of Some Introduced Rubber Tree (Hevea brasiliensis) Clones Cultivated in Ghana(2018-12) Antwi-Wiredu, A.; Amiteye, S.; Asare, A.T.; Kusi-Adjei, R.; Diawuoh, R.G.; Aryee, C.O.; Klu, G.Y.P.Purpose: Genetic makers are indispensable in the genetic quantification and characterization of plant species of which rubber tree is no exception. Markers have been used severally in genetic identification and diversity analysis of rubber tree species. However, in Ghana genetic diversity and relatedness among introduced rubber tree species are limited. The study is aimed to use microsatellite markers to assess the genetic variability and evaluate genetic relationships among clones of Hevea brasiliensis cultivated in Ghana. Research Method: Genomic DNA was extracted from fresh rubber leaves following the Cetyltrimethyl Ammonium Bromide (CTAB) protocol. The five (5) different SSR primers used for the analysis were hmct1, hmac4, hmct5, gSSR212 and gSSR194. Findings: The five SSR markers showed some degree of relatedness among the rubber clones which suggested genetic similarity (IRCA317-5, IRCA41-2, IRCA331-6, IRCA230-4, IRCA109-3, and B8-23) and diversity (K2-18, IRCA840-7, PB217-8, PB217-10, and IRCA317-16) among the clones irrespective of their geographical area of collection. Research Limitation: The unavailability of polivinylpolipyrolidon (PVPP) and polyacrylamide gel (PAGE). Original Value: The results from the study depict that relatively some rubber clones of interest could be selected for future breeding and propagation programmes in Ghana.Item Yellow Maize Breeding For Higher Beta-Carotene: Farmers’ Perceptions Of Vitamin-A Deficiency Consequences And Readiness To Grow Improved Varieties In Ghana(Afr. J. Food Agric. Nutr. Dev, 2023) Ansah, G.; Amoatey, H.M.; Amiteye, S.; et alVitamin A deficiency poses serious health concerns among the Ghanaian populace, particularly children and pregnant women. The production and consumption of yellow maize, which contains pro-vitamin A carotenoids such as beta carotene, is therefore encouraged. Consequently, yellow maize consumption is fast gaining acceptance. However, in the near future comensurate increases in production must be achieved to ensure food security. In this regard, the aims of the study were to: evaluate farmer willingness to cultivate yellow maize varieties with enhanced beta carotene content, ascertain extent of farmers’ knowledge on production constraints and mitigation, and evaluate farmers’ perception on nutritional benefits of yellow maize and the effects of vitamin A deficiency. The survey was conducted between January and May 2018 and it covered four districts representing the forest and guinea savanna ecological zones in Ghana. In each district, five communities with 10 participants each were covered. Most yellow maize farmers (78 %) were between 15 and 55 years, 42.5 % of the farmers had no formal education, 29 % had secondary education and only 8 % had tertiary education. The main challenges facing yellow maize (YM) farmers were pest control and low yield due to erratic rainfall. Majority (70 %) of the farmers had heard about vitamin A deficiency and also noticed stunted growth, mental retardation and poor eye sight in their communities but did not link such symptoms to vitamin A deficiency. Most respondents (70 %) were not aware that yellow maize contains beta carotene. Majority (70 %) of the respondents opined that improvement in the beta carotene content of yellow maize will greatly help to drastically minimize vitamin A deficiency and enhance food security. The information elicited from farmers will guide future development of yellow maize varieties for improved production and nutrition.