The discovery, distribution, and evolution of viruses associated with drosophila melanogaster

Simple item page
dc.contributor.authorWebster, C.L.
dc.contributor.authorWaldron, F.M.
dc.contributor.authorRobertson, S.
dc.contributor.authorCrowson, D.
dc.contributor.authorFerrari, G.
dc.contributor.authorQuintana, J.F.
dc.contributor.authorBrouqui, J.-M.
dc.contributor.authorBayne, E.H.
dc.contributor.authorLongdon, B.
dc.contributor.authorBuck, A.H.
dc.contributor.authorLazzaro, B.P.
dc.contributor.authorAkorli, J.
dc.contributor.authorHaddrill, P.R.
dc.contributor.authorObbard, D.J.
dc.date.accessioned2019-02-15T09:59:53Z
dc.date.available2019-02-15T09:59:53Z
dc.date.issued2015-07
dc.description.abstractDrosophila melanogaster is a valuable invertebrate model for viral infection and antiviral immunity, and is a focus for studies of insect-virus coevolution. Here we use a metagenomic approach to identify more than 20 previously undetected RNA viruses and a DNA virus associated with wild D. melanogaster. These viruses not only include distant relatives of known insect pathogens but also novel groups of insect-infecting viruses. By sequencing virus-derived small RNAs, we show that the viruses represent active infections of Drosophila. We find that the RNA viruses differ in the number and properties of their small RNAs, and we detect both siRNAs and a novel miRNA from the DNA virus. Analysis of small RNAs also allows us to identify putative viral sequences that lack detectable sequence similarity to known viruses. By surveying >2,000 individually collected wild adult Drosophila we show that more than 30% of D. melanogaster carry a detectable virus, and more than 6% carry multiple viruses. However, despite a high prevalence of the Wolbachia endosymbiont— which is known to be protective against virus infections in Drosophila—we were unable to detect any relationship between the presence of Wolbachia and the presence of any virus. Using publicly available RNA-seq datasets, we show that the community of viruses in Drosophila laboratories is very different from that seen in the wild, but that some of the newly discovered viruses are nevertheless widespread in laboratory lines and are ubiquitous in cell culture. By sequencing viruses from individual wild-collected flies we show that some viruses are shared between D. melanogaster and D. simulans. Our results provide an essential evolutionary and ecological context for host–virus interaction in Drosophila, and the newly reported viral sequences will help develop D. melanogaster further as a model for molecular and evolutionary virus research. © 2015 Webster et al.en_US
dc.identifier.otherhttps://doi.org/10.1371/journal.pbio.1002210
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/27552
dc.language.isoenen_US
dc.publisherPLoS Biologyen_US
dc.subjectAntidepressanten_US
dc.subjectBiomarker panelen_US
dc.subjectGABA-glutamateen_US
dc.subjectGenomics-proteomicsen_US
dc.subjectImmune-inflammation-redoxen_US
dc.subjectKynureninecytokineen_US
dc.subjectNeurotransmittersen_US
dc.subjectNitric oxideen_US
dc.subjectSchizophreniaen_US
dc.titleThe discovery, distribution, and evolution of viruses associated with drosophila melanogasteren_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
The discovery, distribution, and evolution of viruses associated with drosophila melanogaster.PDF
Size:
3.27 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.6 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Parasitology Department