Genetic diversity and population structure analysis of Ghanaian and exotic cassava accessions using simple sequence repeat (SSR) markers

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dc.contributor.authorAsante, I.K.
dc.contributor.authorAdjebeng-Danquah, J.
dc.contributor.authorManu-Aduening, J.
dc.contributor.authorAgyare, R.Y.
dc.contributor.authorGracen, V.
dc.contributor.authorOffei, S.K.
dc.date.accessioned2020-03-09T10:41:52Z
dc.date.available2020-03-09T10:41:52Z
dc.date.issued2020-01-31
dc.descriptionResearch Articleen_US
dc.description.abstractGenetic diversity is fundamentally important in crop improvement and provides plants with the capacity to meet the demands of changing environments. This work was carried out to assess the diversity and the extent of genetic relatedness among a number of assembled cassava (Manihot esculenta Crantz) accessions. We conducted a microsatellite marker analysis of 89 cassava accessions collected from Ghanaian and exotic sources. These accessions were assayed using 35 simple sequence repeat (SSR) markers. A total of 167 alleles were detected from 35 polymorphic markers with an average of 4.77 alleles per locus. High allelic frequency was detected across the accessions, ranging from 0.32 to 0.99 with an average of 0.62 per marker. Observed heterozygosity ranged from 0.03 - 0.97 across the accessions. Polymorphism information content (PIC) ranged from 0.03 to 0.78 with a mean of 0.45, indicating high level of polymorphism across the accessions. Comparatively, higher number of alleles, gene diversity and observed heterozygosity were detected among the local accessions compared with the exotic accessions indicating rich genetic diversity among them. Population structure analysis based on STRUCTURE identified two subpopulations and a large number of admixtures. Cluster analysis based on the neighbour joining algorithim further separated the collection into seven sub-groupings irrespective of geographical origin. This indicates the possible sharing of common genomic regions occurring across the accessions. High allelic frequency differences and levels of heterozygosity were observed among the germplasm. These findings indicated significant genetic variability in the germplasm to warrant selection.en_US
dc.description.sponsorshipJ. Adjebeng-Danquah was supported by the West Africa Centre for Crop Improvement (WACCI) PhD program, which received funding from The Generation Challenge Programme (GCP) and the Alliance for a Green Revolution in Africa (AGRA).en_US
dc.identifier.otherhttps://doi.org/10.1016/j.heliyon.2019.e03154
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/35148
dc.language.isoenen_US
dc.publisherHeliyonen_US
dc.relation.ispartofseries6;1
dc.subjectAgricultureen_US
dc.subjectEnvironmental scienceen_US
dc.subjectMolecular markersen_US
dc.subjectGenetic variabilityen_US
dc.subjectManihot esculenta Crantzen_US
dc.subjectHeterozygosityen_US
dc.titleGenetic diversity and population structure analysis of Ghanaian and exotic cassava accessions using simple sequence repeat (SSR) markersen_US
dc.typeArticleen_US

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