Development of Cooperative Primer-Based Real-Time PCR Assays for the Detection of Plasmodium malariae and Plasmodium ovale

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dc.contributor.authorAnsah, F.
dc.contributor.authorSuurbaar, J.
dc.contributor.authorDarko, D.
dc.contributor.authorAnabire, N.G.
dc.contributor.authorBlankson, S.O.
dc.contributor.authorDomson, B.K.S.
dc.contributor.authorSoulama, A.
dc.contributor.authorKpasra, P.
dc.contributor.authorChirawurah, J.D.
dc.contributor.authorAmenga-Etego, L.
dc.contributor.authorKanyong, P.
dc.contributor.authorAwandare, G.A.
dc.contributor.authorAniweh, Y.
dc.date.accessioned2021-10-28T09:35:56Z
dc.date.available2021-10-28T09:35:56Z
dc.date.issued2021
dc.descriptionResearch Articleen_US
dc.description.abstractPlasmodium malariae and Plasmodium ovale are increasingly gaining public health attention as the global transmission of falciparum malaria is decreasing. However, the absence of reliable Plasmodium species-specific detection tools has hampered accurate diagnosis of these minor Plasmodium species. In this study, SYBR Greenebased real-time PCR assays were developed for the detection of P. malariae and P. ovale using cooperative primers that significantly limit the formation and propagation of primersdimers. Both the P. malariae and P. ovale cooperative primer-based assays had at least 10-fold lower detection limit compared with the corresponding conventional primer-based assays. More important, the cooperative primer-based assays were evaluated in a cross-sectional study using 560 samples obtained from two health facilities in Ghana. The prevalence rates of P. malariae and P. ovale among the combined study population were 18.6% (104/560) and 5.5% (31/560), respectively. Among the Plasmodium-positive cases, P. malariae and P. ovale mono-infections were 3.6% (18/499) and 1.0% (5/ 499), respectively, with the remaining being co-infections with Plasmodium falciparum. The study demonstrates the public health importance of including detection tools with lower detection limits in routine diagnosis and surveillance of nonfalciparum species. This will be necessary for comprehensively assessing the effectiveness of malaria interventions and control measures aimed toward global malaria elimination. (en_US
dc.identifier.otherhttps://doi.org/10.1016/j.jmoldx.2021.07.022
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/36904
dc.language.isoenen_US
dc.publisherThe Journal of Molecular Diagnosticsen_US
dc.subjectPlasmodium malariaeen_US
dc.subjectPlasmodium ovaleen_US
dc.subjectfalciparum malariaen_US
dc.titleDevelopment of Cooperative Primer-Based Real-Time PCR Assays for the Detection of Plasmodium malariae and Plasmodium ovaleen_US
dc.typeArticleen_US

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