Comparison of real-time pcr and kato smear microscopy for the detection of hookworm infections in three consecutive faecal samples from schoolchildren in ghana

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dc.contributor.authorvan Mens, S.P.
dc.contributor.authorAryeetey, Y.
dc.contributor.authorYazdanbakhshc, M.
dc.contributor.authorvan Lieshoutc, L.
dc.contributor.authorBoakyec, D.
dc.contributor.authorVerweij, J.J.
dc.date.accessioned2018-12-17T09:56:40Z
dc.date.available2018-12-17T09:56:40Z
dc.date.issued2013-02
dc.description.abstractBackground: The classic technique used to detect hookworm infections in population-based surveys is microscopic examination of Kato thick smears of multiple faecal samples per person as variation in soil-transmitted helminth egg output is common. Methods: As an alternative to this time-consuming and logistically difficult procedure, a PCR-based method to detect hookworm infections was evaluated. Faecal samples collected from 65 Ghanaian school children during February-June 2006 were examined using both techniques. Results: Thirty-one children with ahookworm infection were detected by Kato examination of three faecal samples compared with 30 children detected by PCR of a single faecal sample and 39 detected by PCR of three faecal samples. Conclusion: PCR provides a sensitive alternative to the conventional microscopic detection of hookworm infections. © Royal Society of Tropical Medicine and Hygiene 2013. All rights reserved.en_US
dc.identifier.otherDOI: 10.1093/trstmh/trs094
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/26441
dc.language.isoenen_US
dc.publisherTransactions of the Royal Society of Tropical Medicine and Hygieneen_US
dc.subjectGhanaen_US
dc.subjectHookworm infectionen_US
dc.subjectMicroscopyen_US
dc.subjectReal-time PCRen_US
dc.titleComparison of real-time pcr and kato smear microscopy for the detection of hookworm infections in three consecutive faecal samples from schoolchildren in ghanaen_US
dc.typeArticleen_US

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