Comparison of real-time pcr and kato smear microscopy for the detection of hookworm infections in three consecutive faecal samples from schoolchildren in ghana
dc.contributor.author | van Mens, S.P. | |
dc.contributor.author | Aryeetey, Y. | |
dc.contributor.author | Yazdanbakhshc, M. | |
dc.contributor.author | van Lieshoutc, L. | |
dc.contributor.author | Boakyec, D. | |
dc.contributor.author | Verweij, J.J. | |
dc.date.accessioned | 2018-12-17T09:56:40Z | |
dc.date.available | 2018-12-17T09:56:40Z | |
dc.date.issued | 2013-02 | |
dc.description.abstract | Background: The classic technique used to detect hookworm infections in population-based surveys is microscopic examination of Kato thick smears of multiple faecal samples per person as variation in soil-transmitted helminth egg output is common. Methods: As an alternative to this time-consuming and logistically difficult procedure, a PCR-based method to detect hookworm infections was evaluated. Faecal samples collected from 65 Ghanaian school children during February-June 2006 were examined using both techniques. Results: Thirty-one children with ahookworm infection were detected by Kato examination of three faecal samples compared with 30 children detected by PCR of a single faecal sample and 39 detected by PCR of three faecal samples. Conclusion: PCR provides a sensitive alternative to the conventional microscopic detection of hookworm infections. © Royal Society of Tropical Medicine and Hygiene 2013. All rights reserved. | en_US |
dc.identifier.other | DOI: 10.1093/trstmh/trs094 | |
dc.identifier.uri | http://ugspace.ug.edu.gh/handle/123456789/26441 | |
dc.language.iso | en | en_US |
dc.publisher | Transactions of the Royal Society of Tropical Medicine and Hygiene | en_US |
dc.subject | Ghana | en_US |
dc.subject | Hookworm infection | en_US |
dc.subject | Microscopy | en_US |
dc.subject | Real-time PCR | en_US |
dc.title | Comparison of real-time pcr and kato smear microscopy for the detection of hookworm infections in three consecutive faecal samples from schoolchildren in ghana | en_US |
dc.type | Article | en_US |
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