Genetic Analysis of QTL for Resistance to Maize Lethal Necrosis in Multiple Mapping Populations
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Date
2019-12-26
Journal Title
Journal ISSN
Volume Title
Publisher
genes
Abstract
Maize lethal necrosis (MLN) occurs when maize chlorotic mottle virus (MCMV) and
sugarcane mosaic virus (SCMV) co-infect maize plant. Yield loss of up to 100% can be experienced
under severe infections. Identification and validation of genomic regions and their flanking markers
can facilitate marker assisted breeding for resistance to MLN. To understand the status of previously
identified quantitative trait loci (QTL)in diverse genetic background, F3 progenies derived from
seven bi-parental populations were genotyped using 500 selected kompetitive allele specific PCR
(KASP) SNPs. The F3 progenies were evaluated under artificial MLN inoculation for three seasons.
Phenotypic analyses revealed significant variability (P 0.01) among genotypes for responses to
MLN infections, with high heritability estimates (0.62 to 0.82) for MLN disease severity and AUDPC
values. Linkage mapping and joint linkage association mapping revealed at least seven major QTL
(qMLN3_130 and qMLN3_142, qMLN5_190 and qMLN5_202, qMLN6_85 and qMLN6_157 qMLN8_10
and qMLN9_142) spread across the 7-biparetal populations, for resistance to MLN infections and
were consistent with those reported previously. The seven QTL appeared to be stable across genetic
backgrounds and across environments. Therefore, these QTL could be useful for marker assisted
breeding for resistance to MLN.
Description
Research Article
Keywords
multiple population, linkage mapping, JLAM, QTL, validation, genomic prediction, maize lethal necrosis