Genotypic and phenotypic diversity of Mycobacterium tuberculosis complex genotypes prevalent in West Africa

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dc.contributor.authorOsei-Wusu, S.
dc.contributor.authorOtchere, I.D.
dc.contributor.authorMorgan, P.
dc.contributor.authorMusah, A.B.
dc.contributor.authorSiam, I.M.
dc.contributor.authorAsandem, D.
dc.contributor.authorAfum, T.
dc.contributor.authorAsare, P.
dc.contributor.authorAsante-Poku, A.
dc.contributor.authorKusi, K.A.
dc.contributor.authorGagneux, S.
dc.contributor.authorYeboah-Manu, D.
dc.date.accessioned2021-11-15T11:58:43Z
dc.date.available2021-11-15T11:58:43Z
dc.date.issued2021
dc.descriptionResearch Articleen_US
dc.description.abstractFindings from previous comparative genomics studies of the Mycobacterium tuberculosis complex (MTBC) suggest genomic variation among the genotypes may have phenotypic implications. We investigated the diversity in the phenotypic profiles of the main prevalent MTBC genotypes in West Africa. Thirty-six whole genome sequenced drug susceptible MTBC isolates belonging to lineages 4, 5 and 6 were included in this study. The isolates were phenotypically characterized for urease activity, tween hydrolysis, Thiophen-2- Carboxylic Acid Hydrazide (TCH) susceptibility, nitric oxide production, and growth rate in both liquid (7H9) and solid media (7H11 and Lo¨wenstein–Jensen (L-J)). Lineage 4 isolates showed the highest growth rate in both liquid (p = 0.0003) and on solid (L-J) media supplemented with glycerol (p<0.001) or pyruvate (p = 0.005). L6 isolates optimally utilized pyruvate compared to glycerol (p<0.001), whereas L5 isolates grew similarly on both media (p = 0.05). Lineage 4 isolates showed the lowest average time to positivity (TTP) (p = 0.01; Average TTP: L4 = 15days, L5 = 16.7days, L6 = 29.7days) and the highest logCFU/mL (p = 0.04; average logCFU/mL L4 = 5.9, L5 = 5.0, L6 = 4.4) on 7H11 supplemented with glycerol, but there was no significant difference in growth on 7H11 supplemented with pyruvate (p = 0.23). The highest release of nitrite was recorded for L5 isolates, followed by L4 and L6 isolates. However, the reverse was observed in the urease activity for the lineages. All isolates tested were resistant to TCH except for one L6 isolate. Comparative genomic analyses revealed several mutations that might explain the diverse phenotypic profiles of these isolates. Our findings showed significant phenotypic diversity among the MTBC lineages used for this study.en_US
dc.identifier.citatione0255433. https://doi.org/10.1371/journal. pone.0255433en_US
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/37114
dc.language.isoenen_US
dc.publisherPLOS ONEen_US
dc.subjectGenotypicen_US
dc.subjectMycobacterium tuberculosisen_US
dc.subjectWest Africaen_US
dc.subjectgenotypesen_US
dc.subjectgenomic variationen_US
dc.titleGenotypic and phenotypic diversity of Mycobacterium tuberculosis complex genotypes prevalent in West Africaen_US
dc.typeArticleen_US

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West African Centre for Cell Biology of Infectious Pathogens