Signalling and Bioactive Metabolites from Streptomyces sp. RK44

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dc.contributor.authorKyeremeh, K.
dc.contributor.authorFang, Q.
dc.contributor.authorMaglangit, F.
dc.contributor.authorWu, L.
dc.contributor.authorEbel, R.
dc.contributor.authorAndersen, J.H.
dc.contributor.authorAnnang, F.
dc.contributor.authorPérez-Moreno, G.
dc.contributor.authorReyes, F.
dc.contributor.authorDeng, H.
dc.date.accessioned2020-03-10T12:19:51Z
dc.date.available2020-03-10T12:19:51Z
dc.date.issued2020-01-22
dc.descriptionResearch Articleen_US
dc.description.abstractStreptomyces remains one of the prolific sources of structural diversity, and a reservoir to mine for novel natural products. Continued screening for new Streptomyces strains in our laboratory led to the isolation of Streptomyces sp. RK44 from the underexplored areas of Kintampo waterfalls, Ghana, Africa. Preliminary screening of the metabolites from this strain resulted in the characterization of a new 2-alkyl-4-hydroxymethylfuran carboxamide (AHFA) 1 together with five known compounds, cyclo-(L-Pro-Gly) 2, cyclo-(L-Pro-L-Phe) 3, cyclo-(L-Pro-L-Val) 4, cyclo-(L-Leu-Hyp) 5, and deferoxamine E 6. AHFA 1, a methylenomycin (MMF) homolog, exhibited anti-proliferative activity (EC50 = 89.6 M) against melanoma A2058 cell lines. This activity, albeit weak is the first report amongst MMFs. Furthermore, the putative biosynthetic gene cluster (ahfa) was identified for the biosynthesis of AHFA 1. DFO-E 6 displayed potent anti-plasmodial activity (IC50 = 1.08 M) against P. falciparum 3D7. High-resolution electrospray ionization mass spectrometry (HR ESIMS) and molecular network assisted the targeted-isolation process, and tentatively identified six AHFA analogues, 7–12 and six siderophores 13–18.en_US
dc.description.sponsorshipQ.F. is grateful to the University of Aberdeen Elphinstone Scholarship and Scottish Funding Council/ScotCHEM for financial support through the PEER/PERCE Funding. FM thanks the University of the Philippines for the Faculty, Reps and Sta Development Program (FRAS DP) for the PhD grant fellowship. HD and KK thank the financial supports of Leverhulme Trust-Royal Society Africa award (AA090088) and the jointly funded UK Medical Research Council-UK Department for International Development (MRC/DFID) Concordat agreement African Research Leaders Award (MR/S00520X/1).en_US
dc.identifier.otherdoi:10.3390/molecules25030460
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/35197
dc.language.isoenen_US
dc.publisherMoleculesen_US
dc.relation.ispartofseries25;460
dc.subjectAHFAen_US
dc.subjectmethylenomycinen_US
dc.subjectMMFsen_US
dc.subjectsignalling moleculesen_US
dc.subjectStreptomyces sp.en_US
dc.subjectRK44en_US
dc.subjectanticanceren_US
dc.subjectantimalariaen_US
dc.titleSignalling and Bioactive Metabolites from Streptomyces sp. RK44en_US
dc.typeArticleen_US

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