Biosynthesis of Neocarazostatin A Reveals the Sequential Carbazole Prenylation and Hydroxylation in the Tailoring Steps
dc.contributor.author | Huang, S. | |
dc.contributor.author | Elsayed, S.S. | |
dc.contributor.author | Lv, M. | |
dc.contributor.author | Tabudravu, J. | |
dc.contributor.author | Rateb, E.M. | |
dc.contributor.author | Gyampoh, R. | |
dc.contributor.author | Kyeremeh, K. | |
dc.contributor.author | Ebel, R. | |
dc.contributor.author | Jaspars, M. | |
dc.contributor.author | Deng, Z. | |
dc.contributor.author | Yu, YI. | |
dc.contributor.author | Deng, H. | |
dc.date.accessioned | 2018-08-31T12:43:52Z | |
dc.date.available | 2018-08-31T12:43:52Z | |
dc.date.issued | 2015-12 | |
dc.description.abstract | Neocarazostatin A (NZS) is a bacterial alkaloid with promising bioactivities against free radicals, featuring a tricyclic carbazole nucleus with a prenyl moiety at C-6 of the carbazole ring. Here, we report the discovery and characterization of the biosynthetic pathway of NZS through genome mining and gene inactivation. The in vitro assays characterized two enzymes: NzsA is a P450 hydroxylase and NzsG is a new phytoene-synthase-like prenyltransferase (PTase). This is the first reported native PTase that specifically acts on the carbazole nucleus. Finally, our in vitro reconstituted experiment demonstrated a coupled reaction catalyzed by NzsG and NzsA tailoring the NZS biosynthesis. | en_US |
dc.identifier.citation | Huang et al., 2015,Biosynthesis of Neocarazostatin A Reveals the Sequential Carbazole Prenylation and Hydroxylation in the Tailoring Steps Chemistry & Biology 22, 1633–1642 | en_US |
dc.identifier.other | http://dx.doi.org/10.1016/j.chembiol.2015.10.012 | |
dc.identifier.uri | http://ugspace.ug.edu.gh/handle/123456789/23905 | |
dc.language.iso | en | en_US |
dc.publisher | Chemistry & Biology | en_US |
dc.subject | Neocarazostatin A (NZS) | en_US |
dc.subject | bacterial alkaloid | en_US |
dc.subject | tricyclic carbazole nucleus | en_US |
dc.subject | carbazole ring | en_US |
dc.subject | prenyl moiety at C-6 | en_US |
dc.subject | Biosynthesis | en_US |
dc.subject | Hydroxylation | en_US |
dc.subject | Sequential | en_US |
dc.subject | Carbazole Prenylation | en_US |
dc.title | Biosynthesis of Neocarazostatin A Reveals the Sequential Carbazole Prenylation and Hydroxylation in the Tailoring Steps | en_US |
dc.type | Article | en_US |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- Huang-S_Biosynthesis-of-Neocarazostatin-A-Reveals-the-Sequential-Carbazole-Prenylation-and-Hydroxylation-in-the-Tailoring-StepsOpen-Access_2015.pdf
- Size:
- 2.11 MB
- Format:
- Adobe Portable Document Format
- Description:
License bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- license.txt
- Size:
- 1.6 KB
- Format:
- Item-specific license agreed upon to submission
- Description: