Biosynthesis of Neocarazostatin A Reveals the Sequential Carbazole Prenylation and Hydroxylation in the Tailoring Steps

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dc.contributor.authorHuang, S.
dc.contributor.authorElsayed, S.S.
dc.contributor.authorLv, M.
dc.contributor.authorTabudravu, J.
dc.contributor.authorRateb, E.M.
dc.contributor.authorGyampoh, R.
dc.contributor.authorKyeremeh, K.
dc.contributor.authorEbel, R.
dc.contributor.authorJaspars, M.
dc.contributor.authorDeng, Z.
dc.contributor.authorYu, YI.
dc.contributor.authorDeng, H.
dc.date.accessioned2018-08-31T12:43:52Z
dc.date.available2018-08-31T12:43:52Z
dc.date.issued2015-12
dc.description.abstractNeocarazostatin A (NZS) is a bacterial alkaloid with promising bioactivities against free radicals, featuring a tricyclic carbazole nucleus with a prenyl moiety at C-6 of the carbazole ring. Here, we report the discovery and characterization of the biosynthetic pathway of NZS through genome mining and gene inactivation. The in vitro assays characterized two enzymes: NzsA is a P450 hydroxylase and NzsG is a new phytoene-synthase-like prenyltransferase (PTase). This is the first reported native PTase that specifically acts on the carbazole nucleus. Finally, our in vitro reconstituted experiment demonstrated a coupled reaction catalyzed by NzsG and NzsA tailoring the NZS biosynthesis.en_US
dc.identifier.citationHuang et al., 2015,Biosynthesis of Neocarazostatin A Reveals the Sequential Carbazole Prenylation and Hydroxylation in the Tailoring Steps Chemistry & Biology 22, 1633–1642en_US
dc.identifier.otherhttp://dx.doi.org/10.1016/j.chembiol.2015.10.012
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/23905
dc.language.isoenen_US
dc.publisherChemistry & Biologyen_US
dc.subjectNeocarazostatin A (NZS)en_US
dc.subjectbacterial alkaloiden_US
dc.subjecttricyclic carbazole nucleusen_US
dc.subjectcarbazole ringen_US
dc.subjectprenyl moiety at C-6en_US
dc.subjectBiosynthesisen_US
dc.subjectHydroxylationen_US
dc.subjectSequentialen_US
dc.subjectCarbazole Prenylationen_US
dc.titleBiosynthesis of Neocarazostatin A Reveals the Sequential Carbazole Prenylation and Hydroxylation in the Tailoring Stepsen_US
dc.typeArticleen_US

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