Host Genetics Influences HIV Reservoir Persistence, Treatment Outcomes And CCR5 Expression In Patients On Long-Term Antiretroviral Therapy In Ghana

Abstract

Objective: This study investigated influence of cysteine-cysteine chemokines (CCR5, CCR5-59029A/G, CCR2), and stoma cell derived factor 1 (SDF1); as AIDS restriction genes (ARGs), on treatment outcomes among people with HIV (PWH) on long term antiretroviral therapy (ART) in Ghana.Methods: This cross-sectional study was conducted among 167 PWH and receiving ART in Accra, Ghana. Genomic DNA from PBMCs was used to genotype CCR5-Δ32, CCR2-V64I, CCR5 A59029G, and SDF1-3′A variants using polymerase chain reaction-restriction fragment length polymorphism, CCR5 expression was estimated by RT-qPCR.Results: All participants carried the wild-type CCR5 genotype. No AIDS Restriction Genes was associated with virologic or immunological outcomes (p>0.05). However, individuals with the CCR5 A59029G GG genotype exhibited significantly higher proviral DNA levels than those with the AA genotype (p < 0.05), and the GG genotype independently predicted higher proviral DNA burden in multivariable analysis (β = 0.840, p = 0.011).Conclusion: No ARG variant was significantly associated with virologic or immunological outcomes. However, CCR5 A59029G was associated with differences in proviral DNA burden and CCR5 mRNA expression, suggesting a potential role in HIV reservoir dynamics during suppressive ART.