Allelic diversity of S-RNase alleles in diploid potato species

dc.contributor.authorDzidzienyo, D.K.
dc.contributor.authorBryan, G.J.
dc.contributor.authorWilde, G.
dc.contributor.authorRobbins, T.P.
dc.date.accessioned2019-02-12T16:28:10Z
dc.date.available2019-02-12T16:28:10Z
dc.date.issued2016-10
dc.description.abstractKey message: The S-ribonuclease sequences of 16 S-alleles derived from diploid types of Solanum are presented. A phylogenetic analysis and partial phenotypic analysis support the conclusion that these are functional S-alleles. Abstract: S-Ribonucleases (S-RNases) control the pistil specificity of the self-incompatibility (SI) response in the genus Solanum and several other members of the Solanaceae. The nucleotide sequences of S-RNases corresponding to a large number of S-alleles or S-haplotypes have been characterised. However, surprisingly, few S-RNase sequences are available for potato species. The identification of new S-alleles in diploid potato species is desirable as these stocks are important sources of traits such as biotic and abiotic resistance. S-RNase sequences are reported here from three distinct diploid types of potato: cultivated Solanum tuberosum Group Phureja, S. tuberosum Group Stenotomum, and the wild species Solanum okadae. Partial S-RNase sequences were obtained from pistil RNA by RT-PCR or 3′RACE (Rapid Amplification of cDNA Ends) using a degenerate primer. Full-length sequences were obtained for two alleles by 5′RACE. Database searches with these sequences identified 16 S-RNases in total, all of which are novel. The sequence analysis revealed all the expected features of functional S-RNases. Phylogenetic analysis with selected published S-RNase and S-like-RNase sequences from the Solanaceae revealed extensive trans-generic evolution of the S-RNases and a clear distinction from S-like-RNases. Pollination tests were used to confirm the self-incompatibility status and cross-compatibility relationships of the S. okadae accessions. All the S. okadae accessions were found to be self-incompatible as expected with crosses amongst them exhibiting both cross-compatibility and semi-compatibility consistent with the S-genotypes determined from the S-RNase sequence data. The progeny analysis of four semi-compatible crosses examined by allele-specific PCR provided further confirmation that these are functional S-RNases. © 2016, The Author(s).en_US
dc.identifier.citationDzidzienyo, D.K., Bryan, G.J., Wilde, G. et al. Theor Appl Genet (2016) 129: 1985. https://doi.org/10.1007/s00122-016-2754-7en_US
dc.identifier.otherVolume 129, Issue 10, pp 1985–2001
dc.identifier.otherhttps://doi.org/10.1007/s00122-016-2754-7
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/27466
dc.language.isoenen_US
dc.publisherTheoretical and Applied Geneticsen_US
dc.subjectPotato Germplasmen_US
dc.subjectCompatibility Relationshipen_US
dc.subjectRace Cloningen_US
dc.subjectPrimary Structural Featureen_US
dc.subjectJames Hutton Instituteen_US
dc.titleAllelic diversity of S-RNase alleles in diploid potato speciesen_US
dc.typeArticleen_US

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