Prevalence of hepatitis G virus and characterization of viral genome in Ghana.

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dc.contributor.authorSaito, T.
dc.contributor.authorIshikawa, K.
dc.contributor.authorOsei-Kwasi, M.
dc.contributor.authorKaneko, T.
dc.contributor.authorBrandful, J.A.M.
dc.contributor.authorNuvor, V.
dc.contributor.authorAidoo, S.
dc.contributor.authorAmpofo, W.
dc.contributor.authorApeagyei, F.A.
dc.contributor.authorAnsah, J.E.
dc.contributor.authorAdu-Sarkodie, Y.
dc.contributor.authorNkrumah, F.K.
dc.contributor.authorAbe, K.
dc.date.accessioned2013-06-21T15:26:53Z
dc.date.accessioned2017-10-16T13:15:02Z
dc.date.available2013-06-21T15:26:53Z
dc.date.available2017-10-16T13:15:02Z
dc.date.issued1999
dc.description.abstractThe prevalence of hepatitis G virus (HGV) infection was investigated in 85 human immunodeficiency virus (HIV)-infected and 30 uninfected individuals' sera obtained from Ghanaians. HGV RNA in serum was identified by a nested reverse transcription polymerase chain reaction (RT-PCR) using primers derived from the 5′-noncoding region. We also tested for hepatitis C virus by nested RT-PCR and for hepatitis B surface antigen (HBsAg) by passive hemagglutination method. HGV RNA was detected in 17 of 85 (20%) HIV sero-positive and three of 30 (10%) sero-negative Ghanaians, respectively. The prevalence of HGV infection was much greater than hepatitis C (0.9%) and hepatitis B virus (7.8%) infections in the present study. Ninety four percent of HGV infected patients were seronegative for hepatitis B and C virus infections. The nine different Ghanaian isolates in the 5′-untranslated region of the HGV genome had one nucleotide deletion at the same position when compared with other HGV isolates. Phylogenetic analysis showed that all Ghanaian isolates belonged to type 1 (West Africa type) of the HGV genotypes. Moreover, we determined nearly full-length nucleotide sequence of the HGV genome (denoted HGV-GA128) recovered from a Ghanaian infected with HIV. The HGV-GA128 was composed of 9231 nucleotides and had a single open reading frame, encoding 2843 amino acid residues. This isolate differed from previously reported HGV/GBV-C isolates by 10–15% of the nucleotide sequence and 2–5% of the amino acid sequence. Our data indicate a high prevalence of HGV, especially genotype 1, in Ghana.en_US
dc.identifier.citationSaito, T., Ishikawa, K. -., Osei-Kwasi, M., Kaneko, T., Brandful, J. A. M., Nuvor, V., . . . Abe, K. (1999). Prevalence of hepatitis G virus and characterization of viral genome in Ghana. Hepatology Research, 13(3), 221-231.en_US
dc.identifier.urihttp://197.255.68.203/handle/123456789/3876
dc.language.isoenen_US
dc.publisherHepatology Researchen_US
dc.subjectFull-length sequence of Ghanaian HGV isolate (HGV-GA128)en_US
dc.subjectGhanaen_US
dc.subjectHepatitis G virus (HGV) genomeen_US
dc.subjectHGV genotypesen_US
dc.titlePrevalence of hepatitis G virus and characterization of viral genome in Ghana.en_US
dc.typeArticleen_US

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