Comparative analysis of asexual and sexual stage Plasmodium falciparum development in different red blood cell types

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dc.contributor.authorAmoah, L.E.
dc.contributor.authorAcquah, F.K.
dc.contributor.authorNyarko, P.B.
dc.contributor.authorCudjoe, E.
dc.contributor.authorDonu, D.
dc.contributor.authorAyanful‑Torgby, R.
dc.contributor.authorSey, F.
dc.contributor.authorWilliamson, K.C.
dc.contributor.authorAwandare, G.A.
dc.date.accessioned2020-07-09T10:15:25Z
dc.date.available2020-07-09T10:15:25Z
dc.date.issued2020-06-05
dc.descriptionResearch Articleen_US
dc.description.abstractBackground: Red blood cell (RBC) polymorphisms are suggested to influence the course of Plasmodium falciparum malaria. Whereas some variants have been found to be protective, others have been found to enhance parasite development. This study evaluated the effect of variant haemoglobin (Hb) and ABO blood groups on P. falciparum merozoite invasion, multiplication rates as well as gametocyte development. Methods: Approximately 2.5 mL of venous blood was collected from each participant. Flow cytometry was used to determine the in vitro merozoite invasion rates of NF54 parasites into the blood of 66 non-parasitaemic individuals with variant Hb genotypes (HbSS, HbSC) and blood groups (A, B, O), which were then compared with invasion into HbAA blood. The ex vivo asexual parasite multiplication and gametocyte production rates of parasites from 79 uncomplicated malaria patients with varying Hb genotypes (HbAS, HbAC and HbAA) were also estimated using microscopy. Results: Merozoite invasion rates were significantly reduced by about 50% in RBCs containing HbSS and HbSC relative to HbAA cells. The presence of blood group O and B reduced the invasion rates of HbSS by about 50% and 60%, respectively, relative to HbSC but the presence of blood group A removed the inhibitory effect of HbSS. The initial parasite densities in uncomplicated malaria patients with Hb genotypes HbAS and HbAC cells were similar but significantly lower than those with genotype HbAA. The ex vivo parasite multiplication rate, gametocytaemia and gametocyte conversion rates followed a similar trend but did not reach statistical significance (p > 0.05). Conclusions: Parasite invasion rate into erythrocytes is dependent on both erythrocyte blood group antigen and haemoglobin genotype as blood group O and B provided protection via reduced merozoite invasion in RBCs containing HbSS relative to HbSC. Regardless of haemoglobin type, greater than 70% malaria patients had circulating ring stage parasites that differentiated into stage II gametocytes in 4 days.en_US
dc.description.sponsorshipWest African Centre for Cell Biology of Infectious Pathogens (WACCBIP) (ACE02-WACCBIP) and NIH Grant # AI069314.en_US
dc.identifier.citationAmoah, L.E., Acquah, F.K., Nyarko, P.B. et al. Comparative analysis of asexual and sexual stage Plasmodium falciparum development in different red blood cell types. Malar J 19, 200 (2020). https://doi.org/10.1186/s12936-020-03275-9en_US
dc.identifier.otherhttps://doi.org/10.1186/s12936-020-03275-9
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/35504
dc.language.isoenen_US
dc.publisherMalaria Journalen_US
dc.relation.ispartofseries19;200
dc.subjectHaemoglobinopathiesen_US
dc.subjectMalariaen_US
dc.subjectGametocyteen_US
dc.subjectMerozoiteen_US
dc.subjectRed blood cell (RBC)en_US
dc.subjectpolymorphismsen_US
dc.subjectPlasmodium falciparumen_US
dc.titleComparative analysis of asexual and sexual stage Plasmodium falciparum development in different red blood cell typesen_US
dc.typeArticleen_US

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