Marmesin isolated from Celtis durandii Engl. root bioactive fraction inhibits β-hematin formation and contributes to antiplasmodial activity
dc.contributor.author | Ezenyi, I.C. | |
dc.contributor.author | Chirawurah, J.D. | |
dc.contributor.author | Erhunse, N. | |
dc.contributor.author | Agrawal, P. | |
dc.contributor.author | Sahal, D. | |
dc.contributor.author | Ijoli, J.O. | |
dc.date.accessioned | 2023-08-23T12:31:44Z | |
dc.date.available | 2023-08-23T12:31:44Z | |
dc.date.issued | 2023 | |
dc.description | Research Article | en_US |
dc.description.abstract | Ethnopharmacological relevance: Malaria is a leading cause of death in many developing countries, especially in sub-Saharan Africa. Nigeria is endowed with an abundance of medicinal plants, many of which are used to treat malaria. Celtis durandii Engl. is one such plant used as a traditional antimalarial remedy in southeast Nigeria. However, its antiplasmodial potential is poorly explored. Aim of the study: The study aimed at identifying the antiplasmodial components of C. durandii root extract through antiplasmodial activity-guided fractionation. Materials and methods: Dichloromethane/methanol mixture extract (1:1 v/v) of C. durandii root was prepared and partitioned against water to obtain the organic phase, which was further separated by column chromatography into nine (C1 – C9) fractions. The antiplasmodial activity was evaluated by in vitro screening of the different fractions against drug-sensitive and drug-resistant Plasmodium falciparum strains. Further purification of the active column fractions resulted in a potent anti-Plasmodial compound that was subsequently investigated for its effect on β-hematin formation. Additionally, the isolated compound was characterized and identified as marmesin using mass spectrometry and nuclear magnetic resonance spectroscopy. Results: Celtis durandii root extract exhibited promising antiplasmodial activity {IC50 (μg/ml) 5.92, 6.04, and 6.92} against PfW2mef, PfINDO, and Pf3D7 respectively. Pooled fractions with good antiplasmodial activity {IC50 (μg/ml) Pf3D7: 3.99; PfINDO: 2.24} and selectivity for the parasites (SI: 21) yielded a compound that was fourteen-fold potent in antiplasmodial activity against Pf3D7(IC50: 0.28 μg/ml). It also inhibited β-hematin formation with an IC50 = 150 μM. Further studies using spectral data, literature, and chemical databases identified the purified compound as marmesin. Conclusion: This work has demonstrated that Celtis durandii root extract has good antiplasmodial activity against drug-sensitive and drug-resistant P. falciparum. The inhibition of β-hematin formation by marmesin accounts in part for this activity. | en_US |
dc.identifier.other | https://doi.org/10.1016/j.jep.2023.116804 | |
dc.identifier.uri | http://ugspace.ug.edu.gh:8080/handle/123456789/39810 | |
dc.language.iso | en | en_US |
dc.publisher | Journal of Ethnopharmacology | en_US |
dc.subject | Antiplasmodial | en_US |
dc.subject | Celtis durandii | en_US |
dc.subject | Malaria | en_US |
dc.subject | Marmesin | en_US |
dc.title | Marmesin isolated from Celtis durandii Engl. root bioactive fraction inhibits β-hematin formation and contributes to antiplasmodial activity | en_US |
dc.type | Article | en_US |
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