Colorimetric Detection of Plasmodium vivax in Urine Using MSP10 Oligonucleotidesand Gold Nanoparticles
Date
2016-10-05
Authors
Alnasser, Y.
Ferradas, C.
Clark, T.
Calderon, M.
Gurbillon, A.
Gamboa, D.
McKakpo, U.S.
Quakyi, I.A.
Bosompem, K.M.
Sullivan, D.J.
Journal Title
Journal ISSN
Volume Title
Publisher
PLOS Neglected Tropical Diseases
Abstract
Plasmodium vivax is the most prevalent cause of human malaria in the world and can lead
to severe disease with high potential for relapse. Its genetic and geographic diversities
make it challenging to control. P. vivax is understudied and to achieve control of malaria in
endemic areas, a rapid, accurate, and simple diagnostic tool is necessary. In this pilot
study, we found that a colorimetric system using AuNPs and MSP10 DNA detection in urine
can provide fast, easy, and inexpensive identification of P. vivax. The test exhibited promising
sensitivity (84%), high specificity (97%), and only mild cross-reactivity with P. falciparum
(21%). It is simple to use, with a visible color change that negates the need for a spectrometer,
making it suitable for use in austere conditions. Using urine eliminates the need for finger-
prick, increasing both the safety profile and patient acceptance of this model.
Description
Journal Article
Keywords
Colorimetric Detection, Plasmodium vivax, Urine, MSP10 Oligonucleotides, Gold Nanoparticles
Citation
Alnasser Y, Ferradas C, Clark T, Calderon M, Gurbillon A, Gamboa D, et al. (2016) Colorimetric Detection of Plasmodium vivax in Urine Using MSP10 Oligonucleotides and Gold Nanoparticles. PLoS Negl Trop Dis 10(10): e0005029. doi:10.1371/journal.pntd.0005029