Genetic relationship between bacteria isolated from intraoperative air samples and surgical site infections at a major teaching hospital in Ghana

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dc.contributor.authorBediako-Bowan, A.
dc.contributor.authorStauning, M.A.
dc.contributor.authorBjerrum, S.
dc.contributor.authorAndersen, L.P.
dc.contributor.authorAndreu-Sanchez, S.
dc.contributor.authorLabi, A-K.
dc.contributor.authorKurtzhals, J.A.L.
dc.contributor.authorMarvig, R.L.
dc.contributor.authorOpintan, J.A.
dc.date.accessioned2020-01-30T15:55:24Z
dc.date.available2020-01-30T15:55:24Z
dc.date.issued2019-11-16
dc.descriptionResearch Articleen_US
dc.description.abstractBackground: In low- and middle-income countries (LMICs) the rate of surgical site infections (SSI) is high, leading to negative patient outcomes and excess healthcare costs. A causal relationship between airborne bacteria in the operating room and SSI has not been established, at a molecular or genetic level. We studied the relationship between intraoperative airborne bacteria and bacteria causing SSI in an LMIC. Methods: Active air sampling using a portable impactor was performed during clean or clean-contaminated elective surgical procedures. Active patient follow-up consisting of phone calls and clinical examinations was performed 3, 14 and 30 days after surgery. Bacterial isolates recovered from SSI and air samples were compared by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) identification, ribotyping, whole genome sequencing (WGS), and metagenomic analysis. Results: Of 128 included patients, 116 (91%) completed follow-up and 11 (9%) developed SSI. Known pathogenic bacteria were isolated from intraoperative air samples in all cases with SSI. A match between air and SSI isolates was found by MALDI-TOF in eight cases. Matching ribotypes were found in six cases and in one case both WGS and metagenomic analysis showed identity between air- and SSI-isolates. Conclusion: The study showed high levels of intraoperative airborne bacteria, an SSIrate of 9% and a genetic link between intraoperative airborne bacteria and bacteriaen_US
dc.description.sponsorshipDANIDA (ref. A30025 and 16-P01-GHA), Augustinusfonden (ref. 17-0408), Rigshospitalets Forskningsfond, University of Copenhagen (ref. A5287), Dansk Tennisfond (ref.13.02.90), Knud Højgaards fond (ref. 16-01-0991), Nordea Fonden (ref. 01-2016-001569) and Oticon Fonden (ref. 16-1716). R.L.M. is supported by the Danish National Research Foundation (grant number 126).en_US
dc.identifier.citationStauning MA et al., Genetic relationship between bacteria isolated from intraoperative air samples and surgical site infections at a major teaching hospital in Ghana, Journal of Hospital Infection, https://doi.org/10.1016/j.jhin.2019.11.007en_US
dc.identifier.otherhttps://doi.org/10.1016/j.jhin.2019.11.007
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/34710
dc.language.isoenen_US
dc.publisherJournal of Hospital Infectionen_US
dc.relation.ispartofseries103;3
dc.subjectSurgical site infectionsen_US
dc.subjectAirborne bacteriaen_US
dc.subjectLow- and middle-incomeen_US
dc.subjectcountriesen_US
dc.subjectStaphylococcus aureusen_US
dc.titleGenetic relationship between bacteria isolated from intraoperative air samples and surgical site infections at a major teaching hospital in Ghanaen_US
dc.typeArticleen_US

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