Genetic relationship between bacteria isolated from intraoperative air samples and surgical site infections at a major teaching hospital in Ghana
dc.contributor.author | Bediako-Bowan, A. | |
dc.contributor.author | Stauning, M.A. | |
dc.contributor.author | Bjerrum, S. | |
dc.contributor.author | Andersen, L.P. | |
dc.contributor.author | Andreu-Sanchez, S. | |
dc.contributor.author | Labi, A-K. | |
dc.contributor.author | Kurtzhals, J.A.L. | |
dc.contributor.author | Marvig, R.L. | |
dc.contributor.author | Opintan, J.A. | |
dc.date.accessioned | 2020-01-30T15:55:24Z | |
dc.date.available | 2020-01-30T15:55:24Z | |
dc.date.issued | 2019-11-16 | |
dc.description | Research Article | en_US |
dc.description.abstract | Background: In low- and middle-income countries (LMICs) the rate of surgical site infections (SSI) is high, leading to negative patient outcomes and excess healthcare costs. A causal relationship between airborne bacteria in the operating room and SSI has not been established, at a molecular or genetic level. We studied the relationship between intraoperative airborne bacteria and bacteria causing SSI in an LMIC. Methods: Active air sampling using a portable impactor was performed during clean or clean-contaminated elective surgical procedures. Active patient follow-up consisting of phone calls and clinical examinations was performed 3, 14 and 30 days after surgery. Bacterial isolates recovered from SSI and air samples were compared by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) identification, ribotyping, whole genome sequencing (WGS), and metagenomic analysis. Results: Of 128 included patients, 116 (91%) completed follow-up and 11 (9%) developed SSI. Known pathogenic bacteria were isolated from intraoperative air samples in all cases with SSI. A match between air and SSI isolates was found by MALDI-TOF in eight cases. Matching ribotypes were found in six cases and in one case both WGS and metagenomic analysis showed identity between air- and SSI-isolates. Conclusion: The study showed high levels of intraoperative airborne bacteria, an SSIrate of 9% and a genetic link between intraoperative airborne bacteria and bacteria | en_US |
dc.description.sponsorship | DANIDA (ref. A30025 and 16-P01-GHA), Augustinusfonden (ref. 17-0408), Rigshospitalets Forskningsfond, University of Copenhagen (ref. A5287), Dansk Tennisfond (ref.13.02.90), Knud Højgaards fond (ref. 16-01-0991), Nordea Fonden (ref. 01-2016-001569) and Oticon Fonden (ref. 16-1716). R.L.M. is supported by the Danish National Research Foundation (grant number 126). | en_US |
dc.identifier.citation | Stauning MA et al., Genetic relationship between bacteria isolated from intraoperative air samples and surgical site infections at a major teaching hospital in Ghana, Journal of Hospital Infection, https://doi.org/10.1016/j.jhin.2019.11.007 | en_US |
dc.identifier.other | https://doi.org/10.1016/j.jhin.2019.11.007 | |
dc.identifier.uri | http://ugspace.ug.edu.gh/handle/123456789/34710 | |
dc.language.iso | en | en_US |
dc.publisher | Journal of Hospital Infection | en_US |
dc.relation.ispartofseries | 103;3 | |
dc.subject | Surgical site infections | en_US |
dc.subject | Airborne bacteria | en_US |
dc.subject | Low- and middle-income | en_US |
dc.subject | countries | en_US |
dc.subject | Staphylococcus aureus | en_US |
dc.title | Genetic relationship between bacteria isolated from intraoperative air samples and surgical site infections at a major teaching hospital in Ghana | en_US |
dc.type | Article | en_US |
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