Rapid high throughput SYBR green assay for identifying the malaria vectors Anopheles arabiensis, Anopheles coluzzii and Anopheles gambiae s.s. Giles

Simple item page
dc.contributor.authorChabi, J.
dc.contributor.authorVan’t Hof, A.
dc.contributor.authorN’dri, L.K.
dc.contributor.authorDatsomor, A.
dc.contributor.authorOkyere, D.
dc.contributor.authorNjoroge, H.
dc.contributor.authorPipini, D.
dc.contributor.authorHadi, M.P.
dc.contributor.authorDe Souza, D.K.
dc.contributor.authorSuzuki, T.
dc.contributor.authorDadzie, S.K.
dc.contributor.authorJamet, H.P.
dc.date.accessioned2019-05-16T16:42:39Z
dc.date.available2019-05-16T16:42:39Z
dc.date.issued2019-04
dc.description.abstractThe Anopheles gambiae sensu lato species complex consists of a number of cryptic species with different habitats and behaviours. These morphologically indistinct species are identified by chromosome banding. Several molecular diagnostic techniques for distinguishing between An. coluzzii and An. gambiae are still under improvement. Although, the current SINE method for identification between An. coluzzii and An. gambiae works reliably, this study describes a refinement of the SINE method to increase sensitivity for identification of An. coluzzii, An. gambiae and An. arabiensis based on amplicon dissociation curve characteristics. Field-collected samples, laboratory-reared colonies and crossed specimens of the two species were used for the design of the protocol. An. gambiae, An. coluzzii, and hybrids of the two species were sampled from Ghana and An. arabiensis from Kenya. Samples were first characterised using conventional SINE PCR method, and further assayed using SYBR green, an intercalating fluorescent dye. The three species and hybrids were clearly differentiated using the melting temperature of the dissociation curves, with derivative peaks at 72˚C for An. arabiensis, 75˚C for An. gambiae and 86˚C for An. coluzzii. The hybrids (An. gambiae / An. coluzzii) showed both peaks. This work is the first to describe a SYBR green real time PCR method for the characterization of An. arabiensis, An. gambiae and An. coluz-zii and was purposely designed for basic melt-curve analysis (rather than high-resolution melt-curve) to allow it to be used on a wide range of real-time PCR machines.en_US
dc.identifier.citationChabi J, Van’t Hof A, N’dri LK, Datsomor A, Okyere D, Njoroge H, et al. (2019) Rapid high throughput SYBR green assay for identifying the malaria vectors Anopheles arabiensis, Anopheles coluzzii and Anopheles gambiae s.s. Giles. PLoS ONE 14(4): e0215669. https://doi.org/10.1371/journal.pone.0215669en_US
dc.identifier.otherhttps://doi.org/10.1371/journal.pone.0215669
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/30097
dc.language.isoenen_US
dc.publisherPLoS ONEen_US
dc.titleRapid high throughput SYBR green assay for identifying the malaria vectors Anopheles arabiensis, Anopheles coluzzii and Anopheles gambiae s.s. Gilesen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Rapid high throughput SYBR green assay for identifying the malaria vectors Anopheles arabiensis, Anopheles coluzzii and Anopheles gambiae s.s. Giles.pdf
Size:
885.31 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.6 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Parasitology Department