Elevated IL-17 levels in semi-immune anaemic mice infected with Plasmodium berghei ANKA

Simple item page
dc.contributor.authorHelegbe, G.K.
dc.contributor.authorHuy, N.T.
dc.contributor.authorYanagi, T.
dc.contributor.authorShuaibu, M.N.
dc.contributor.authorKikuchi, M.
dc.contributor.authorCherif, M.S.
dc.contributor.authorHirayama, K.
dc.date.accessioned2018-10-08T15:24:31Z
dc.date.available2018-10-08T15:24:31Z
dc.date.issued2018-04
dc.description.abstractBackground: Alterations in inflammatory cytokines and genetic background of the host contribute to the outcome of malaria infection. Despite the promising protective role of IL-17 in infections, little attention is given to further understand its importance in the pathogenesis of severe malaria anaemia in chronic/endemic situations. The objective of this study, therefore, was to evaluate IL-17 levels in anaemic condition and its association with host genetic factors. Methods: Two mice strains (Balb/c and CBA) were crossed to get the F1 progeny, and were (F1, Balb/c, CBA) taken through 6 cycles of Plasmodium berghei (ANKA strain) infection and chloroquine/pyrimethamine treatment to generate semi-immune status. Cytokine levels and kinetics of antibody production, CD4+CD25+T regulatory cells were evaluated by bead-based multiplex assay kit, ELISA and FACs, respectively. Results: High survival with high Hb loss at significantly low parasitaemia was observed in Balb/c and F1. Furthermore, IgG levels were two times higher in Balb/c, F1 than CBA. While CD4+CD25+ Treg cells were lower in CBA; IL-4, IFN-γ, IL-12α and IL-17 were significantly higher (p < 0.05) in Balb/c, F1. Conclusions: In conclusion, elevated IL-17 levels together with high IL-4, IL-12α and IFN-γ levels may be a marker of protection, and the mechanism may be controlled by host factor (s). Further studies of F2 between the F1 and Balb/c will be informative in evaluating if these genes are segregated or further apart. © 2018 The Author(s).en_US
dc.identifier.otherdoi:10.1186/s12936-018-2257-x
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/24526
dc.language.isoenen_US
dc.publisherBioMed Central Ltd.en_US
dc.subjectAnaemiaen_US
dc.subjectIL-17en_US
dc.subjectPlasmodium berghei ANKAen_US
dc.subjectSemi-immune miceen_US
dc.titleElevated IL-17 levels in semi-immune anaemic mice infected with Plasmodium berghei ANKAen_US
dc.typeArticleen_US

Files

License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.6 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Department of Biochemistry, Cell and Molecular Biology