A shared Asian origin of the triple-mutant DHFR allele in plasmodium falciparum from sites across Africa.
dc.contributor.author | Maiga, O. | |
dc.contributor.author | Djimde, A.A. | |
dc.contributor.author | Hubert, V. | |
dc.contributor.author | Renard, E. | |
dc.contributor.author | Aubouy, A. | |
dc.contributor.author | Kironde, F. | |
dc.contributor.author | Nsimba, B. | |
dc.contributor.author | Koram, K.A. | |
dc.contributor.author | Doumbo, O.K. | |
dc.contributor.author | Le Bras, J. | |
dc.contributor.author | Clain, J. | |
dc.date.accessioned | 2013-06-17T15:38:14Z | |
dc.date.accessioned | 2017-10-16T13:08:09Z | |
dc.date.available | 2013-06-17T15:38:14Z | |
dc.date.available | 2017-10-16T13:08:09Z | |
dc.date.issued | 2007-07-01 | |
dc.description.abstract | Background. Usefulness of sulfadoxine-pyrimethamine as first-line therapy for uncomplicated Plasmodium falciparum malaria and intermittent preventive treatment in pregnancy throughout sub-Saharan Africa is compromised by the spread of dhfr alleles associated with pyrimethamine resistance. A predominant haplotype associated with the N51I+C59R+S108N triple-mutant dhfr allele has been reported recently in 4 African countries. A more comprehensive picture of the evolution of this mutant allele in Africa is lacking. Methods. Seventy-five P. falciparum isolates carrying the wild-type dhfr allele and 204 carrying the triple-mutant dhfr allele from 11 African countries were selected. The genetic diversity of the chromosomes bearing these alleles was analyzed with 4 microsatellite markers closely linked to the dhfr gene. Results. Seventy-three different 4-locus haplotypes carrying the wild-type dhfr allele were found. By contrast, 175 (85%) of 204 isolates carrying the triple-mutant dhfr allele shared a unique haplotype, identical to the one identified in Thailand. For the remaining triple-mutant isolates and one isolate with the quadruple-mutant dhfr allele (N51I+C59R+S108N+I164L), haplotypes were closely related to the predominant haplotype by mutation or recombination. Conclusions. Migration of parasites carrying an ancestral triple-mutant dhfr allele drives the spread of dhfr alleles associated with pyrimethamine resistance throughout West and Central Africa. © 2007 by the Infectious Diseases Society of America. All rights reserved. | en_US |
dc.identifier.citation | Maïga, O., Djimdé, A. A., Hubert, V., Renard, E., Aubouy, A., Kironde, F., . . . Clain, J. (2007). A shared Asian origin of the triple-mutant dhfr allele in plasmodium falciparum from sites across africa. Journal of Infectious Diseases, 196(1), 165-172. | en_US |
dc.identifier.issn | 00221899 | |
dc.identifier.uri | http://197.255.68.203/handle/123456789/3323 | |
dc.subject | EMTREE medical terms: adult; Africa; allele; article; Asian; chromosome; dhfr gene; female; gene; gene locus; gene mutation; genetic recombination; genetic variability; haplotype; heterozygote; human; infection prevention; major clinical study; malaria; male; microsatellite marker; molecular evolution; mutant; nonhuman; parasite isolation; Plasmodium falciparum; priority journal; protozoal genetics; Thailand; wild type | en_US |
dc.title | A shared Asian origin of the triple-mutant DHFR allele in plasmodium falciparum from sites across Africa. | en_US |
dc.type | Article | en_US |