Genome sequence of Mycobacterium yongonense RT 955-2015 isolate from a patient misdiagnosed with multidrug-resistant tuberculosis: First clinical detection in Tanzania

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dc.contributor.authorMnyambwa, N.P.
dc.contributor.authorKim, D.-J.
dc.contributor.authorNgadaya, E.
dc.contributor.authorChun, J.
dc.contributor.authorHa, S.-M.
dc.contributor.authorPetrucka, P.
dc.contributor.authorAddo, K.K.
dc.contributor.authorKazwala, R.R.
dc.contributor.authorMfinanga, S.G.
dc.date.accessioned2019-02-05T08:24:26Z
dc.date.available2019-02-05T08:24:26Z
dc.date.issued2018-06
dc.description.abstractBackground: Mycobacterium yongonense is a recently described novel species belonging to Mycobacterium avium complex, which is the most prevalent aetiology of non-tuberculous mycobacteria associated with pulmonary infections, and poses tuberculosis diagnostic challenges in high-burden, resource-constrained settings. Methods: Whole genome shotgun sequencing and comparative microbial genomic analyses were used to characterize the isolate from a patient diagnosed with multidrug-resistant tuberculosis (MDR-TB) after relapse. Results: The genome sequence of the first case of M. yongonense (M. yongonense RT 955-2015) in Tanzania is presented. Sequence analysis revealed that the RT 955-2015 strain had a high similarity to M. yongonense 05-1390(T) (98.74%) and Mycobacterium chimaera DSM 44623(T) (98%). Its 16S rRNA showed similarity to Mycobacterium paraintracellulare KCTC 290849(T) (100%), Mycobacterium intracellulare ATCC 13950(T) (100%), M. chimaera DSM 44623(T) (99.9%), and M. yongonense 05-1390(T) (98%). The strain exhibited a substantially different rpoB sequence to that of M. yongonense 05-1390 (95.16%), but closely related to that of M. chimaera DSM 44623(T) (99.86%), M. intracellulare ATCC 13950(T), (99.53%), and M. paraintracellulare KCTC 290849(T) (99.53%). Conclusions: In light of the OrthoANI algorithm and phylogenetic analysis, it was concluded that the isolate was M. yongonense Type II genotype, which is an indication that the patient was misdiagnosed with TB/MDR-TB and received inappropriate treatment. © 2018 The Author(s)en_US
dc.identifier.otherhttps://doi.org/10.1016/j.ijid.2018.04.796
dc.identifier.otherVolume 71, Pages 82-88
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/27225
dc.language.isoenen_US
dc.publisherInternational Journal of Infectious Diseasesen_US
dc.subjectMultidrug-resistant tuberculosis (MDR-TB)en_US
dc.subjectMycobacterium avium complex (MAC)en_US
dc.subjectMycobacterium yongonenseen_US
dc.subjectNon-tuberculous mycobacteria (NTM)en_US
dc.subjectTanzaniaen_US
dc.titleGenome sequence of Mycobacterium yongonense RT 955-2015 isolate from a patient misdiagnosed with multidrug-resistant tuberculosis: First clinical detection in Tanzaniaen_US
dc.typeArticleen_US

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