DNA in situ hybridization on polytene chromosomes of Simulium sanctipauli at loci relevant to insecticide resistance

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dc.contributor.authorBoakye, D.A.
dc.contributor.authorCornel, A.J.
dc.contributor.authorMeredith, S.E.
dc.contributor.authorBrakefield, P.M.
dc.contributor.authorCollins, F.H.
dc.date.accessioned2019-02-21T13:34:01Z
dc.date.available2019-02-21T13:34:01Z
dc.date.issued2000-06
dc.description.abstractA DNA technique for in situ hybridization developed by Kumar and Collins (1994) for use on polytene chromosomes of adult Anopheles mosquitoes (Diptera: Culicidae) was modified for use with Simulium larval salivary gland chromosomes (Diptera: Simuliidae). Cloned fragments of several Simulium genes (coding for aspartate amino transferase, cytochrome P450 and DNA polymerase) were successfully mapped physically by assigning specific band locations in Simulim sanctipauli V. and D. This represents the first attempt at locating genes beyond the resolution of linkage to inversions in any blackfly species.en_US
dc.identifier.otherhttps://doi.org/10.1046/j.1365-2915.2000.00230.x
dc.identifier.otherVolume 14, Issue 2,Pages 217-222
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/28090
dc.language.isoenen_US
dc.publisherMedical and Veterinary Entomologyen_US
dc.subjectAspartate amino transferaseen_US
dc.subjectChromosome mappingen_US
dc.subjectCote d'Ivoireen_US
dc.subjectCytochrome P450en_US
dc.subjectDNA hybridizationen_US
dc.subjectGhanaen_US
dc.subjectPolymerase chain reactionen_US
dc.subjectPolytene chromosomesen_US
dc.subjectS. sanctipaulien_US
dc.subjectSimulium damnosum complexen_US
dc.titleDNA in situ hybridization on polytene chromosomes of Simulium sanctipauli at loci relevant to insecticide resistanceen_US
dc.typeArticleen_US

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