Transport of 14C-deferiprone in normal, thalassaemic and sickle red blood cells
| dc.contributor.author | Shalev, O. | |
| dc.contributor.author | Hileti, D. | |
| dc.contributor.author | Nortey, P. | |
| dc.contributor.author | Hebbel, R.P. | |
| dc.contributor.author | Hoffbrand, V.A. | |
| dc.date.accessioned | 2013-06-19T10:38:52Z | |
| dc.date.accessioned | 2017-10-16T12:35:13Z | |
| dc.date.available | 2013-06-19T10:38:52Z | |
| dc.date.available | 2017-10-16T12:35:13Z | |
| dc.date.issued | 1999-06 | |
| dc.description.abstract | The transport of deferiprone (L1) in normal (N), sickle (S) and thalassaemic (T) red blood cells (RBC) was determined by incubation with 14C-L1 at 37 degrees C. Following incubation with 0.5 mM 14C-L1 for 4 h, the intracellular concentration of L1 in T RBC was 3 times higher than was found extracellularly. In contrast, no concentration gradient across N and S RBC membranes was detected. Efflux studies showed that T RBC released only 17 +/- 2% of 14C-L1 into the extracellular space. We hypothesize that L1 accumulation in T RBC results from their high content of chelatable iron and formation of large, hydrophilic L1-Fe(III) complexes trapped within the cytosol. | en_US |
| dc.identifier.citation | Shalev, O., Hileti, D., Nortey, P., Hebbel, R. P., & Hoffbrand, V. A. (1999). Transport of 14C-deferiprone in normal, thalassaemic and sickle red blood cells. British Journal of Haematology, 105(4), 1081-1083 | en_US |
| dc.identifier.uri | http://197.255.68.203/handle/123456789/3628 | |
| dc.language.iso | en | en_US |
| dc.publisher | British Journal of Haematology | en_US |
| dc.title | Transport of 14C-deferiprone in normal, thalassaemic and sickle red blood cells | en_US |
| dc.type | Article | en_US |