Isolation of nontuberculous mycobacteria from the environment of ghanian communities where buruli ulcer is endemic

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dc.contributor.authorAboagye, S.Y.
dc.contributor.authorDanso, E.
dc.contributor.authorAmpah, K.A.
dc.contributor.authorNakobu, Z.
dc.contributor.authorAsare, P.
dc.contributor.authorOtchere, I.D.
dc.contributor.authorRöltgen, K.
dc.contributor.authorYirenya-Tawiah, D.
dc.contributor.authorYeboah-Manu, D.
dc.date.accessioned2019-05-06T17:09:10Z
dc.date.available2019-05-06T17:09:10Z
dc.date.issued2016
dc.description.abstractThis study aimed to isolate nontuberculous mycobacterial species from environmental samples obtained from some selected communities in Ghana. To optimize decontamination, spiked environmental samples were used to evaluate four decontamination solutions and supplemented media, after which the best decontamination solution and media were used for the actual analysis. The isolates obtained were identified on the basis of specific genetic sequences, including heat shock protein 65, IS2404, IS2606, rpoB, and the ketoreductase gene, as needed. Among the methods evaluated, decontamination with 1MNaOH followed by 5% oxalic acid gave the highest rate of recovery of mycobacteria (50.0%) and the lowest rate of contamination (15.6%). The cultivation medium that supported the highest rate of recovery of mycobacteria was polymyxin B-amphotericin B-nalidixic acidtrimethoprim- azlocillin-supplemented medium (34.4%), followed by isoniazid-supplemented medium (28.1%). Among the 139 samples cultivated in the main analysis, 58 (41.7%) yielded mycobacterial growth, 70 (50.4%) had no growth, and 11 (7.9%) had all inoculated tubes contaminated. A total of 25 different mycobacterial species were identified. Fifteen species (60%) were slowly growing (e.g., Mycobacterium ulcerans, Mycobacterium avium, Mycobacterium mantenii, and Mycobacterium malmoense), and 10 (40%) were rapidly growing (e.g., Mycobacterium chelonae, Mycobacterium fortuitum, and Mycobacterium abscessus). The occurrence of mycobacterial species in the various environmental samples analyzed was as follows: soil, 16 species (43.2%); vegetation, 14 species (38.0%); water, 3 species (8.0%); moss, 2 species (5.4%); snail, 1 species (2.7%); fungi, 1 species (2.7%). This study is the first to report on the isolation of M. ulcerans and other medically relevant nontuberculous mycobacteria from different environmental sources in Ghana. © 2016, American Society for Microbiology.en_US
dc.identifier.citationAboagye SY, Danso E, Ampah KA, et al. Isolation of Nontuberculous Mycobacteria from the Environment of Ghanian Communities Where Buruli Ulcer Is Endemic. Appl Environ Microbiol. 2016;82(14):4320–4329. Published 2016 Jun 30. doi:10.1128/AEM.01002-16en_US
dc.identifier.issn992240
dc.identifier.otherdoi.10.1128/AEM.01002-16
dc.identifier.othervol.82(14): 4320–4329.
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/29825
dc.language.isoenen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.titleIsolation of nontuberculous mycobacteria from the environment of ghanian communities where buruli ulcer is endemicen_US
dc.typeArticleen_US

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