Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes

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dc.contributor.authorEcklu-Mensah, G.
dc.contributor.authorOlsen, R.W.
dc.contributor.authorBengtsson, A.
dc.contributor.authorOfori, M.F.
dc.contributor.authorHviid, L.
dc.contributor.authorJensen, A.T.R.
dc.contributor.authorAdams, Y.
dc.date.accessioned2019-06-26T09:34:21Z
dc.date.available2019-06-26T09:34:21Z
dc.date.issued2018-10
dc.description.abstractThe lack of suitable animal models for the study of cytoadhesion of P. falciparum-infected erythrocytes (IEs) has necessitated in vitro studies employing a range of cell lines of either human tumour origin (e.g., BeWo and C32 cells) or non-human origin (e.g., CHO cells). Of the human cells available, many were isolated from adults, or derived from a pool of donors (e.g., HBEC-5i). Here we demonstrate, for the first time, the successful isolation of blood outgrowth endothelial cells (BOECs) from frozen stabilates of peripheral blood mononuclear cells obtained from small-volume peripheral blood samples from paediatric malaria patients. BOECs are a sub-population of human endothelial cells, found within the peripheral blood. We demonstrate that these cells express receptors such as Intercellular Adhesion Molecule 1 (ICAM-1/CD54), Endothelial Protein C Receptor (EPCR/CD201), platelet/endothelial cell adhesion molecule 1 (PECAM-1/CD31), Thrombomodulin (CD141), and support adhesion of P. falciparum IEs.en_US
dc.identifier.citationEcklu-Mensah G, Olsen RW, Bengtsson A, Ofori MF, Hviid L, Jensen ATR, et al. (2018) Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes. PLoS ONE 13(10): e0204177. https://doi.org/10.1371/journal.pone.0204177en_US
dc.identifier.otherhttps://doi.org/10.1371/journal.pone.0204177
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/31036
dc.language.isoenen_US
dc.publisherPLoS ONEen_US
dc.titleBlood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytesen_US
dc.typeArticleen_US

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