Genomic Analysis Of Enterobacteriaceae From Colorectal Cancer Patients At A Tertiary Hospital In Ghana: A Case-Control Study.

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dc.contributor.authorBachelle, S.V.
dc.contributor.authorBah, S.Y.
dc.contributor.authorAddo, R.T.
dc.contributor.authorBediako-Bowan, A.A.A.
dc.contributor.authorEgyir, B.
dc.contributor.authorTsatsu, S.E.
dc.contributor.authoret al.
dc.date.accessioned2025-06-25T15:11:34Z
dc.date.issued2024-10-05
dc.descriptionResearch Article
dc.description.abstractColorectal cancer (CRC) is a severe gastrointestinal cancer and a leading cause of cancer-related deaths in Ghana. The potential role of gut Enterobacteriaceae in the increasing incidence of CRC in Ghana is yet to be thoroughly investigated. In this study, Enterobacteriaceae from CRC patients and healthy control participants were analyzed by whole genome sequencing to identify genomic features that are associated with CRC. Socio-demographic data showed a significant association between age and alcohol consumption and CRC. Escherichia coli was the most abundant Enterobacteriaceae isolated from the study participants and they were predominantly intestinal commensals. Escherichia coli isolates belonging to phylogroup D encoded the highest number of virulence genes. The agn43 and int genes were widespread in Escherichia coli isolates from the CRC patients. Multilocus sequence types of potentially pathogenic Escherichia coli from the CRC patients also encoded genes involved in aggregation, adherence and biofilm formation. The ampC2 and ampH antimicrobial resistance genes were also widespread in the genome of the Escherichia coli isolates. This study highlights the virulence tendencies of Escherichia coli from CRC patients and their ability to transfer virulence determinants to other Enterobacteriaceae residing in the gut.
dc.description.sponsorshipPatient recruitment and initial sample processing were supported by the BANGA-Africa Seed Research Grant from the University of Ghana. Bacterial identification and whole genome sequencing were funded by the SeqAfrica project, which is supported by the UK Department of Health and Social Care's Fleming Fund through UK aid. The views expressed in this publication are those of the authors and do not necessarily reflect those of the UK Department of Health and Social Care or its management agent, Mott MacDonald. The authors are grateful to Christian Owusu-Nyantakyi and Felicia Amoa Owusu at the Bacteriology Department of the Noguchi Me morial Institute For Medical Research for their vital roles in bacterial identification, whole genome sequencing and initial bioinformatic analyses.
dc.identifier.citationBachelle, S. V., Bah, S. Y., Addo, R. T., Bediako-Bowan, A. A., Egyir, B., Tsatsu, S. E., ... & Amarh, V. (2024). Genomic analysis of Enterobacteriaceae from colorectal cancer patients at a tertiary hospital in Ghana: a case-control study. Scientific Reports, 14(1), 23195.
dc.identifier.urihttps://doi.org/10.1038/s41598-024-74299-3
dc.identifier.urihttps://ugspace.ug.edu.gh/handle/123456789/43225
dc.language.isoen
dc.publisherScientific Reports
dc.subjectGenomics
dc.subjectColorectal Cancer
dc.subjectEnterobacteriaceae
dc.subjectVirulence Factors
dc.subjectAntimicrobial Resistance
dc.titleGenomic Analysis Of Enterobacteriaceae From Colorectal Cancer Patients At A Tertiary Hospital In Ghana: A Case-Control Study.
dc.typeArticle

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