Detection of antigens and antibodies in the urine of humans with plasmodium falciparum malaria.

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dc.contributor.authorRodriguez-del Valle, M.
dc.contributor.authorQuakyi, I.A.
dc.contributor.authorAmuesi, J.
dc.contributor.authorQuaye, J.T.
dc.contributor.authorNkrumah, F.K.
dc.contributor.authorTaylor, D.W.
dc.date.accessioned2013-06-21T15:16:52Z
dc.date.accessioned2017-10-16T13:05:58Z
dc.date.available2013-06-21T15:16:52Z
dc.date.available2017-10-16T13:05:58Z
dc.date.issued1991
dc.description.abstractHumans infected with Plasmodium falciparum frequently have elevated levels of proteins in their urine, but it is unclear if any of these proteins are parasite antigens or antimalarial antibodies. To resolve this question, urine samples from malaria patients and controls living in Thailand and Ghana were evaluated. Urine samples from 85% of the patients had elevated protein levels and contained proteins with M(r)s ranging from <29,000 to >224,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Antisera were produced against urine from infected and control subjects. Antisera raised against infected, but not control, urine were positive by indirect immunofluorescence on P. falciparum parasites and immunoprecipitated approximately 12 unique bands from extracts of parasites metabolically labeled with 35S-methionine. These data suggest that a variety of P. falciparum antigens are released into urine during acute infection. It is also likely that anti-P. falciparum antibodies are present in the urine of malaria patients because samples from these patients, but not controls, were positive in indirect immunofluorescence assays and immunoprecipitated at least 19 P. falciparum antigens from extracts of metabolically labeled parasites. The detection of malarial antigens and antibodies in urine may lead to a new approach for the diagnosis of malaria.en_US
dc.description.sponsorshipThis project was supported by the DiaTech Program, P.A.T.H. Funds for collection of the urine samples from Ghana were provided by the International Atomic Energy Agency Research (contract no. 3233/RB, August 1982-July 1983) and in part by the United NationsDevelopment Program/World Bank/World Health Organization Special Program for Research and Training in Tropical Diseases.en_US
dc.identifier.citationRodriguez-del Valle, M., Quakyi, I. A., Amuesi, J., Quaye, J. T., Nkrumah, F. K., & Taylor, D. W. (1991). Detection of antigens and antibodies in the urine of humans with plasmodium falciparum malaria. Journal of Clinical Microbiology, 29(6), 1236-1242.en_US
dc.identifier.urihttp://hinari-gw.who.int/whalecomwww.ncbi.nlm.nih.gov/whalecom0/pmc/articles/PMC269976/
dc.identifier.urihttp://197.255.68.203/handle/123456789/3874
dc.language.isoenen_US
dc.publisherJournal of Clinical Microbiologyen_US
dc.titleDetection of antigens and antibodies in the urine of humans with plasmodium falciparum malaria.en_US
dc.typeArticleen_US

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