Evaluation of two novel Ziehl-Neelsen methods for tuberculosis diagnosis

Simple item page
dc.contributor.authorFrimpong, E.H.
dc.contributor.authorAdukpo, R.
dc.contributor.authorOwusu-Darko, K.
dc.date.accessioned2019-03-21T09:49:20Z
dc.date.available2019-03-21T09:49:20Z
dc.date.issued2005
dc.description.abstractBackground: Currently, the diagnosis of tuberculosis (TB) in Ghana relies on direct sputum smear, Ziehl- Neelsen (ZN) staining method. This method has low sensitivity and poses some health risks. The study was to compare the, direct sputum smear, (ZN) staining method against two newer ZN methods; 1% Sodium hypochlorite (NaOCL)-xylene floatation and 1% NaOCL sedimentation methods, to determine the most sensitive and the safest. Study design: A prospective descriptive study involving 150 adult patients attending Komfo Anokye Teaching Hospital, Kumasi, Ghana suspected of pulmonary tuberculosis, using the three ZN microscopy methods: direct sputum smear, 1% NaOCL sedimentation, and 1% NaOCL-xylene floatation, for the detection of acid fast bacilli (AFB). Sputum culture on Lowenstein-Jensen (LJ) slopes was used as the gold standard for determining the sensitivity and specificity rates. Results: The sensitivity rates of NaOCL sedimentation, NaOCL-xylene floatation and direct smear methods were 77.2%, 71.8% and 66.3% respectively. The specificity rate was 95.9% for all three methods. Whereas the difference between the NaOCL sedimentation and the direct smear methods was statistically significant (P= 0.0446), that between the NaOCL-xylene floatation and direct smear was not (P=0.1788). Conclusion: In spite of the cost of chemicals, the hypochlorite sedimentation method was found to be the most accurate and the safest.en_US
dc.identifier.otherhttp://dx.doi.org/10.4314/wajm.v24i4.28224
dc.identifier.otherVol. 24(4): pp 316-20
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/28759
dc.language.isoenen_US
dc.publisherWest African Journal of Medicineen_US
dc.subjectLaboratory infectionen_US
dc.subjectSputum microscopyen_US
dc.subjectTuberculosisen_US
dc.titleEvaluation of two novel Ziehl-Neelsen methods for tuberculosis diagnosisen_US
dc.typeArticleen_US

Files

License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.6 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Bacteriology Department