Lack of insertional-deletional polymorphism in a collection of mycobacterium ulcerans isolates from Ghanaian buruli ulcer patients

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dc.contributor.authorKäser, M.
dc.contributor.authorGutmann, O.
dc.contributor.authorHauser, J.
dc.contributor.authorStinear, T.
dc.contributor.authorCole, S.
dc.contributor.authorYeboah-Manu, D.
dc.contributor.authorDernick, G.
dc.contributor.authorCerta, U.
dc.contributor.authorPluschke, G.
dc.date.accessioned2013-06-19T09:50:01Z
dc.date.accessioned2017-10-16T13:06:41Z
dc.date.available2013-06-19T09:50:01Z
dc.date.available2017-10-16T13:06:41Z
dc.date.issued2009
dc.description.abstractMycobacterium ulcerans causes the devastating infectious skin disease Buruli ulcer and has a monomorphic population structure. The resolution of conventional genetic fingerprinting methods is therefore not sufficient for microepidemiological studies aiming to characterize transmission pathways. In a previous comparative genomic hybridization analysis with a microarray covering part of the M. ulcerans genome, we have found extensive insertional-deletional sequence polymorphisms among M. ulcerans isolates of diverse geographic origins that allowed us to distinguish between strains coming from different continents. Since large numbers of insertion sequences are spread over the genome of African M. ulcerans strains, we reasoned that these may drive large sequence polymorphisms in otherwise clonal local mycobacterial populations. In this study, we used a printed DNA microarray covering the whole genome of the Ghanaian M. ulcerans reference strain Agy99 for comparative genomic hybridization. The assay identified multiple regions of difference when DNA of a Japanese M. ulcerans strain was analyzed. In contrast, not a single insertional-deletional genomic variation was found within a panel of disease isolates coming from an area of Ghana where Buruli ulcer is endemic. These results indicate that, despite the expectations deduced from other mycobacterial pathogens, only analyses of single nucleotide polymorphisms will have the potential to differentiate local populations of M. ulcerans.en_US
dc.identifier.citationKäser, M., Gutmann, O., Hauser, J., Stinear, T., Cole, S., Yeboah-Manu, D., . . . Pluschke, G. (2009). Lack of insertional-deletional polymorphism in a collection of mycobacterium ulcerans isolates from Ghanaian buruli ulcer patients. Journal of Clinical Microbiology, 47(11), 3640-3646.en_US
dc.identifier.urihttp://197.255.68.203/handle/123456789/3617
dc.language.isoenen_US
dc.publisherJournal of Clinical Microbiologyen_US
dc.titleLack of insertional-deletional polymorphism in a collection of mycobacterium ulcerans isolates from Ghanaian buruli ulcer patientsen_US
dc.typeArticleen_US

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