Molecular diagnosis of Schistosoma infections in urine samples of school children in Ghana

Simple item page
dc.contributor.authorAryeetey, Y.A.
dc.contributor.authorEssien-Baidoo, S.
dc.contributor.authorLarbi, I.A.
dc.contributor.authorAhmed, K.
dc.contributor.authorAmoah, A.S.
dc.contributor.authorObeng, B.B.
dc.contributor.authorVan Lieshout, L.
dc.contributor.authorYazdanbakhsh, M.
dc.contributor.authorBoakye, D.A.
dc.contributor.authorVerweij, J.J.
dc.date.accessioned2018-12-13T11:27:09Z
dc.date.available2018-12-13T11:27:09Z
dc.date.issued2013-06
dc.description.abstractRecent studies using an internal transcribed spacer (ITS)-based real-time polymerase chain reaction (PCR) for the detection of Schistosoma DNA in urine samples has shown high sensitivity and specificity when performed on controls and known microscopy-positive samples. In this study, using 730 urine samples collected from children in five primary schools from different communities in the Greater Accra region of Ghana, specific detection of Schistosoma DNA showed excellent sensitivity of 100% and 85.2% in urines with > 50 eggs/10 mL urine and ≤ 50 eggs/10 mL of urine, respectively. Additionally, Schistosoma-specific DNA was amplified in 102 of 673 samples in which Schistosoma eggs could not be detected with microscopy. Taking microscopy and/or PCR-positive samples as true positives, the negative predictive value calculated was 94.6-100% for each school sampled as compared with 54.3-95.7% using microscopy. This ITS-based real-time PCR proves to be a powerful tool in epidemiological surveys of schistosomiasis providing more precise and sensitive results than microscopy. Copyright © 2013 by The American Society of Tropical Medicine and Hygiene.en_US
dc.identifier.otherdoi:10.4269/ajtmh.12-0571
dc.identifier.otherVol. 88(6), 2013, pp. 1028–1031
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/26401
dc.language.isoenen_US
dc.publisherAmerican Journal of Tropical Medicine and Hygieneen_US
dc.subjectMolecular diagnosisen_US
dc.subjectSchistosoma infectionsen_US
dc.subjecturineen_US
dc.subjectschool childrenen_US
dc.subjectGhanaen_US
dc.titleMolecular diagnosis of Schistosoma infections in urine samples of school children in Ghanaen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Molecular diagnosis of Schistosoma infections in urine samples of school children in Ghana.pdf
Size:
574.38 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.6 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Parasitology Department