Evolutionary changes between pre- and post-vaccine South African group A G2P[4] rotavirus strains, 2003–2017

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dc.contributor.authorMwangi, P.N.
dc.contributor.authorPage, N.A.
dc.contributor.authorSeheri, M.L.
dc.contributor.authorMphahlele, M.J.
dc.contributor.authorNadan, S.
dc.contributor.authorEsona, M.D.
dc.contributor.authorKumwenda, B.
dc.contributor.authorKamng’ona, A.W.
dc.contributor.authorDonato, C.M.
dc.contributor.authorSteele, D.A.
dc.contributor.authorNdze, V.N.
dc.contributor.authorDennis, F.E.
dc.contributor.authorJere, K.C.
dc.contributor.authorNyaga, M.M.
dc.date.accessioned2022-06-01T15:21:51Z
dc.date.available2022-06-01T15:21:51Z
dc.date.issued2022
dc.descriptionResearch Articleen_US
dc.description.abstractThe transient upsurge of G2P[4] group A rotavirus (RVA) after Rotarix vaccine introduction in several countries has been a matter of concern. To gain insight into the diversity and evolution of G2P[4] strains in South Africa pre and post-RVA vaccination introduction, whole-genome sequencing was performed for RVA positive faecal specimens collected between 2003 and 2017 and samples previously sequenced were obtained from GenBank (n=103; 56 pre- and 47 post-vaccine). Pre-vaccine G2 sequences predominantly clustered within sub- lineage IVa-1. In contrast, post-vaccine G2 sequences clustered mainly within sub-lineage IVa-3, whereby a radical amino acid (AA) substitution, S15F, was observed between the two sub- lineages. Pre-vaccine P[4] sequences predominantly segregated within sub-lineage IVa while post-vaccine sequences clustered mostly within sub-lineage IVb, with a radical AA substitution R162G. Both S15F and R162G occurred outside recognised antigenic sites. The AA residue at position 15 is found within the signal sequence domain of Viral Protein 7 (VP7) involved in translocation of VP7 into endoplasmic reticulum during infection process. The 162 AA residue lies within the hemagglutination domain of Viral Protein 4 (VP4) engaged in interaction with sialic acid- containing structure during attachment to the target cell. Free energy change analysis on VP7 indicated accumulation of stable point mutations in both antigenic and non-antigenic regions. The segregation of South African G2P[4] strains into pre and post-vaccination sub-lineages is likely due to erstwhile hypothesized step-wise lineage/sub-lineage evolution of G2P[4] strains rather than RVA vaccine introduction. Our findings reinforce the need for continuous whole- genome RVA surveillance and investigation of contribution of AA substitutions in understanding the dynamic G2P[4] epidemiology.en_US
dc.identifier.otherDOI 10.1099/mgen.0.000809
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/38115
dc.language.isoenen_US
dc.publisherMicrobiology Societyen_US
dc.subjectG2P[4] group A rotavirus strainsen_US
dc.subjectrotavirusen_US
dc.subjectsub-lineagesen_US
dc.subjectwhole-genome analysisen_US
dc.titleEvolutionary changes between pre- and post-vaccine South African group A G2P[4] rotavirus strains, 2003–2017en_US
dc.typeArticleen_US

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