Identification of lactic acid bacteria isolated during traditional fura processing in Ghana

Simple item page
dc.contributor.authorOwusu-Kwarteng, J.
dc.contributor.authorAkabanda, F.
dc.contributor.authorNielsen, D.S.
dc.contributor.authorTano-Debrah, K.
dc.contributor.authorGlover, R.L.K.
dc.contributor.authorJespersen, L.
dc.date.accessioned2019-01-11T11:34:54Z
dc.date.available2019-01-11T11:34:54Z
dc.date.issued2012-10
dc.description.abstractFura is a millet-based spontaneously fermented dumpling produced and consumed in parts of West Africa, particularly Nigeria, Burkina Faso and Ghana. From eight traditional fura production sites in northern Ghana, 862 lactic acid bacteria were isolated and identified to species level using a combination of genotypic and phenotypic methods including (GTG)5-based PCR fingerprinting and 16S rRNA gene sequencing, multiplex PCR by means of recA gene sequence comparison, conventional morphological characteristics and carbohydrate fermentation profiling. During millet dough fermentation, pH decreased from 5.6-6.4 to 4.1-3.7 and total lactic acid bacteria (LAB) counts increased from 4.4-5.3 to 7.9-9.2 log10 (cfu/g). The initial stages of the fermentation were characterized by co-dominance of homo- and heterofermentative species of Pediococcus acidilactici, Weisella confusa, Lactobacillus fermentum, Lactobacillus reuteri, Lactobacillus salivarius, and Lactobacillus paraplantarum whereas L. fermentum was dominating at the end of the fermentation. L. fermentum was predominant in all fermentations (p < 0.05) and a high uniformity was observed among production sites regarding the dominance of L. fermentum. L. fermentum and W. confusa were isolated in all production sites and almost at all fermentation stages indicating that they are indigenous to traditional fura processing. The other LAB bacteria species which comprised a minor proportion of the total LAB occurred occasionally and in an irregular pattern among the production sites. © 2012 Elsevier Ltd.en_US
dc.identifier.otherVol. 32(1): pp 72-8
dc.identifier.otherDOI: 10.1016/j.fm.2012.04.010
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/26769
dc.language.isoenen_US
dc.publisherFood Microbiologyen_US
dc.subjectFuraen_US
dc.subjectLactic acid bacteriaen_US
dc.subjectMilleten_US
dc.subjectRep-PCRen_US
dc.subjectTraditional processingen_US
dc.titleIdentification of lactic acid bacteria isolated during traditional fura processing in Ghanaen_US
dc.typeArticleen_US

Files

License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.6 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Department of Nutrition and Food Science