Assessment of rDNA IGS as a molecular marker in the Simulium damnosum complex

dc.contributor.authorMorales-Hojas, R.
dc.contributor.authorPost, R.J.
dc.contributor.authorCheke, R.A.
dc.contributor.authorWilson, M.D.
dc.date.accessioned2019-03-04T09:13:58Z
dc.date.available2019-03-04T09:13:58Z
dc.date.issued2002-12
dc.description.abstractFor five cytospecies of the Simulium damnosum Theobald complex of blackflies (Diptera: Simuliidae) from West Africa, both ends of the intergenic spacer region (IGS) of the rDNA have been sequenced with the aim of developing specific molecular markers. No specific differences in these two regions were detected between Simulium sanctipauli V. & D., Simulium sirbanum V. & D., Simulium soubrense V. & D., Simulium squamosum Enderlein and Simulium yahense V. & D., except in the number of A subrepeats at the 5′ end of the IGS (two in S. squamosum and four or five in the others) and in position 310 of the 3′ end (a C in S. squamosum and a G in the others). However, genetic distances within and between species overlapped. These DNA sequences had no strong phylogenetic signal, and the trees obtained were mostly unresolved. Although most sequences from S. squamosum clustered together, a few of them were more similar to those in other cytospecies. These results could be explained either by hybridization with genetic introgression or by ancestral polymorphism and recent speciation.en_US
dc.identifier.otherhttps://doi.org/10.1046/j.1365-2915.2002.00391.x
dc.identifier.otherVol. 16(4): pp 395-403
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/28496
dc.language.isoenen_US
dc.publisherMedical and Veterinary Entomologyen_US
dc.subjectAfricaen_US
dc.subjectETSen_US
dc.subjectHybridizationen_US
dc.subjectIGSen_US
dc.subjectMolecular markersen_US
dc.subjectRecent speciationen_US
dc.subjectRibosomal DNAen_US
dc.subjectSimulium damnosum complexen_US
dc.titleAssessment of rDNA IGS as a molecular marker in the Simulium damnosum complexen_US
dc.typeArticleen_US

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