Evaluating the Effects of Kidney Preservation at 10 ◦C with Hemopure and Sodium Thiosulfate in a Rat Model of Syngeneic Orthotopic Kidney Transplantation
Date
2024
Journal Title
Journal ISSN
Volume Title
Publisher
International Journal of Molecular Sciences
Abstract
Kidney transplantation is preferred for end-stage renal disease. The current gold standard
for kidney preservation is static cold storage (SCS) at 4 ◦C. However, SCS contributes to renal graft
damage through ischemia–reperfusion injury (IRI). We previously reported renal graft protection
after SCS with a hydrogen sulfide donor, sodium thiosulfate (STS), at 4 ◦C. Therefore, this study aims
to investigate whether SCS at 10 ◦C with STS and Hemopure (blood substitute), will provide similar
protection. Using in vitro model of IRI, we subjected rat renal proximal tubular epithelial cells to
hypoxia–reoxygenation for 24 h at 10 ◦C with or without STS and measured cell viability. In vivo, we
preserved 36 donor kidneys of Lewis rats for 24 h in a preservation solution at 10 ◦C supplemented
with STS, Hemopure, or both followed by transplantation. Tissue damage and recipient graft function
parameters, including serum creatinine, blood urea nitrogen, urine osmolality, and glomerular
filtration rate (GFR), were evaluated. STS-treated proximal tubular epithelial cells exhibited enhanced
viability at 10 ◦C compared with untreated control cells (p < 0.05). Also, STS and Hemopure improved
renal graft function compared with control grafts (p < 0.05) in the early time period after the transplant,
but long-term function did not reach significance. Overall, renal graft preservation at 10 ◦C with
STS and Hemopure supplementation has the potential to enhance graft function and reduce kidney
damage, suggesting a novel approach to reducing IRI and post-transplant complications.
Description
Research Article
Keywords
sodium thiosulfate (STS), Hemopure, ischemia–reperfusion injury (IRI
Citation
Abou Taka, M.; Dugbartey, G.J.; Richard-Mohamed, M.; McLeod, P.; Jiang, J.; Major, S.; Arp, J.; O’Neil, C.; Liu, W.; Gabril, M.; et al. Evaluating the Effects of Kidney Preservation at 10 ◦C with Hemopure and Sodium Thiosulfate in a Rat Model of Syngeneic Orthotopic Kidney Transplantation. Int. J. Mol. Sci. 2024, 25, 2210. https://doi.org/ 10.3390/ijms25042210