Optimization of cassava (Manihot esculenta Crantz) grafting technique to enhance its adoption in cassava cultivation
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Date
2024
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MethodsX
Abstract
Grafting techniques have been successfully adopted to improve resistance to biotic and abiotic
stresses, increase yields, fruit quality and study systemic signaling in plants. This technique has
not been fully explored in cassava and there is currently no standardized grafting method for
this species published especially in Africa. This is the first report on cassava grafting protocol in
Africa with valuable advantages including utilizing a cost-effective and environmentally friendly
wooden healing chamber. In this study, we describe an optimized cleft grafting protocol for cas sava utilizing a wooden healing chamber and outline the step-by-step procedure with optimum
conditions to generate a high grafting success rate. Using a top wedge grafting technique with high
reproducibility and success rates, we developed a straightforward and robust grafting protocol for
cassava (M. esculenta) cultivars. Grafting success was recorded and this protocol produced a high
grafting success of 90 % and its reproducibility makes it suitable for mass production thereby
addressing the need for efficient cassava propagation. This grafting protocol requires less special ized equipment and expertise making it more accessible to farmers and researchers with limited
resources to promote the use of grafting for cassava growth, yield improvement and advanced
studies such as systemic long-distance signaling in plants.
• Optimization of cleft grafting method obtains a high success grafting rate of cassava.
• A wooden healing chamber provides a controlled environment for graft healing.
• Promoting cassava grafting; a priority to produce new cultivars and explore breeding research
prospects.
Description
Research Article
Keywords
Grafting, Cassava, Cleft grafting