The bulk fluorescein diacetate assay (FDA) as a technique for evaluating biotic impacts of crude oil to coastal sediments

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dc.contributor.authorMahu, E.
dc.contributor.authorMoore-Hanaway, J.
dc.contributor.authorMaurer, B.
dc.contributor.authorWelschmeyer, N.
dc.contributor.authorCoale, K.H.
dc.date.accessioned2019-06-19T11:12:16Z
dc.date.available2019-06-19T11:12:16Z
dc.date.issued2018-11
dc.description.abstractThis study adapted the bulk fluorescein diacetate (FDA) assay to evaluate the impact of crude oil on biological activity levels in marine sediments. Sediment cores from the Elkhorn Slough, California were incubated with different levels of sweet crude oil (Deepwater Horizon, Macondo Prospect) in the Moss landing Marine Laboratories aquarium facility over a 30-day period. During this period, the amount of fluorescein released, concentration of oxygen in overlying waters, and flux of oxygen into the sediments were monitored. For all treatments a strong negative correlation between added oil and FDA production was observed (p < 0.05). The concentration of oxygen in the first 2 mm of sediment cores decreased linearly with the addition of crude oil and correlated positively with fluorescein released (p < 0.05). A linear increase in sediment oxygen flux was also observed upon crude oil addition. The miniaturized bulk FDA assay could be powerful in evaluating oil spill impacts in coastal systems. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.en_US
dc.identifier.citationMahu, E., Moore-Hanaway, J., Maurer, B. et al. Environ Earth Sci (2018) 77: 751. https://doi.org/10.1007/s12665-018-7945-xen_US
dc.identifier.otherhttps://doi.org/10.1007/s12665-018-7945-x
dc.identifier.urihttp://ugspace.ug.edu.gh/handle/123456789/30971
dc.language.isoenen_US
dc.publisherEnvironmental Earth Sciencesen_US
dc.subjectCrude oil impactsen_US
dc.subjectFluorescein diacetateen_US
dc.subjectMacondo prospecten_US
dc.subjectMarine sedimentsen_US
dc.titleThe bulk fluorescein diacetate assay (FDA) as a technique for evaluating biotic impacts of crude oil to coastal sedimentsen_US
dc.typeArticleen_US

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